[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"health-study-detail:100643224":3},{"organization":4,"outcomesModule":7,"designInfo":17,"detailedDescription":20,"studyPopulation":21,"armGroups":22,"interventions":33,"overallOfficials":43,"centralContacts":48,"locations":54,"responsibleParty":74,"collaborators":16,"id":78,"slug":79,"hasResults":80,"nctId":81,"briefTitle":82,"officialTitle":83,"acronym":84,"eligibilityCriteria":85,"healthyVolunteers":86,"sex":87,"minAge":88,"maxAge":89,"enrollmentInfo":90,"targetDuration":16,"studyType":93,"phases":16,"briefSummary":94,"conditions":95,"keywords":101,"overallStatus":109,"whyStopped":16,"lastUpdateSubmitDate":110,"lastUpdatePostDateStruct":111,"startDateStruct":114,"completionDateStruct":116,"leadSponsor":118,"locationsCount":119},{"fullName":5,"class":6},"Bahcesehir Cyprus University","OTHER",{"primaryOutcomes":8,"secondaryOutcomes":16,"otherOutcomes":16},[9,13],{"measure":10,"description":11,"timeFrame":12},"Between-arm difference in PINK1 and Parkin mitophagy gene expression","Relative expression levels of PINK1 and Parkin mRNA will be quantified via probe-based quantitative reverse transcription PCR (qRT-PCR) from isolated peripheral blood mononuclear cells (PBMCs). Normalization will be performed using the geometric mean of housekeeping genes GAPDH and B2M. Evaluation will focus on the between-arm contrast (Arm A vs. Arm B) of the change metric using relative quantification (2\\^-ΔΔCt).","Change from baseline (Day -3) to Day 30 of the fasting period",{"measure":14,"description":15,"timeFrame":12},"Between-arm difference in mitochondrial DNA copy number (mtCN)","Mitochondrial DNA copy number will be quantified by qPCR targeting the mitochondrial reference region MT-RNR1 (12S rRNA), normalized to the nuclear reference gene B2M. Relative mtCN is calculated using the formula: 2 × (Ct\\_nuclear - Ct\\_mtDNA). The primary evaluation is the between-arm difference in net change at Day 30 to test for a predicted U-shaped cellular trajectory",null,{"allocation":16,"interventionModel":16,"interventionModelDescription":16,"primaryPurpose":16,"observationalModel":18,"timePerspective":19,"maskingInfo":16},"COHORT","PROSPECTIVE","This is a prospective, single-centre, parallel-group, longitudinal observational cohort study conducted across Ramadan 2027. The study aims to evaluate the distinct molecular and metabolic trajectories of intermittent dry fasting compared to standard fluid-permitted time-restricted feeding (TRF).\n\nParticipants will be allocated to one of two parallel groups based on their self-selected, established fasting practices:\n\n* Group A (Ramadan Dry Fasting): Observing complete fluid and nutrient deprivation from dawn (Fajr) to sunset (Maghrib) for 30 consecutive days.\n* Group B (16:8 TRF): Practicing a 16-hour daily fasting window with water\u002Ffluids permitted, observed concurrently during the same calendar period.\n\nParticipants in both groups will be pairwise-matched based on age and BMI at screening. Blood samples will be collected from all participants at five distinct milestones:\n\n* T0: Pre-Ramadan baseline (Day -3)\n* T1: Acute induction phase (Day 7)\n* T2: Mitochondrial turnover phase (Day 14)\n* T3: Saturation plateau \u002F primary endpoint (Day 30)\n* T4: Post-Ramadan recovery phase (Day 44)\n\nPeripheral blood mononuclear cells (PBMCs) and serum will be isolated from the collected blood. Laboratory analyses will measure mitochondrial DNA (mtDNA) copy number changes via qPCR, alongside a quantitative gene expression panel evaluating key regulators of mitophagy (such as PINK1 and Parkin), mitochondrial biogenesis, and general autophagy. Secondary evaluations include routine serum metabolic indicators, inflammatory markers, and dietary monitoring to evaluate the physiological pathways predicted by computational systems pharmacology.","The study population consists of healthy adult volunteers aged 18 to 50 with a BMI between 18.5 and 30.0 kg\u002Fm². The cohort represents individuals who are either observing the religious dry fast of Ramadan or concurrently practicing standard 16:8 time-restricted feeding. Participants are recruited from the academic and local communities of Bahçeşehir Cyprus University and Near East University in Nicosia, Northern Cyprus\\[cite: 1\\]. The sample includes both males and females who do not smoke, have stable sleep-wake cycles, and are free from chronic diseases requiring regular prescription medications\\[cite: 1\\].",[23,28],{"label":24,"type":16,"description":25,"interventionNames":26},"Arm A: Ramadan Dry Fasting","Participants observing complete, daily fluid and nutrient deprivation from dawn (Fajr) to sunset (Maghrib) for 30 consecutive days during Ramadan 2027",[27],"Behavioral: Ramadan Intermittent Dry Fasting (Sawm)",{"label":29,"type":16,"description":30,"interventionNames":31},"Arm B: 16:8 Time-Restricted Feeding","Pairwise-matched control participants practicing a fluid-permitted, 16-hour daily fasting window observed concurrently during the same calendar period",[32],"Behavioral: 16:8 Time-Restricted Feeding (TRF)",[34,39],{"type":35,"name":36,"description":37,"armGroupLabels":38,"otherNames":16},"BEHAVIORAL","Ramadan Intermittent Dry Fasting (Sawm)","Total fluid and nutrient restriction enforced daily from dawn (Fajr) to sunset (Maghrib) for 30 consecutive days. Food and fluid consumption are restricted to the nocturnal period",[24],{"type":35,"name":40,"description":41,"armGroupLabels":42,"otherNames":16},"16:8 Time-Restricted Feeding (TRF)","A 16-hour daily fasting window where calorie-containing foods and beverages are completely restricted. Fluid intake (such as water or uncaloried drinks) is permitted throughout the fasting period",[29],[44],{"name":45,"affiliation":46,"role":47},"Berna Uzun","Operational Research Institute in Healthcare, Near East University, Nicosia, TRNC, Mersin 10, Turkey","STUDY_CHAIR",[49],{"name":50,"role":51,"phone":52,"phoneExt":16,"email":53},"Ibrahim Ibrahim Shuaibu, MD, Msc","CONTACT","+905338730889","ibrahim.shuaibu@baucyprus.edu.tr",[55],{"facility":56,"status":16,"city":57,"state":16,"zip":16,"country":58,"countryCode":59,"cosmosGeoPoint":60,"geoPoint":65,"contacts":66},"Near East University","Nicosia","Cyprus","CY",{"type":61,"coordinates":62},"Point",[63,64],33.35397,35.17284,{"lat":64,"lon":63},[67,71],{"name":68,"role":51,"phone":69,"phoneExt":16,"email":70},"Berna Uzun, Prof.","+90 533 871 98 38","berna.uzun@neu.edu.tr",{"name":72,"role":73,"phone":16,"phoneExt":16,"email":16},"Adanze Cynthia Nge","SUB_INVESTIGATOR",{"type":75,"investigatorFullName":76,"investigatorTitle":77,"investigatorAffiliation":5,"oldNameTitle":16,"oldOrganization":16},"PRINCIPAL_INVESTIGATOR","Ibrahim Ibrahim Shuaibu, MD","Principal Investigator","100643224","mitophagy-and-mitochondrial-dna-dynamics-during-ramadan-dry-fasting-vs-168-time-restricted-feeding-100643224",false,"NCT07638696","Mitophagy and Mitochondrial DNA Dynamics During Ramadan Dry Fasting vs 16:8 Time-Restricted Feeding","Mitophagic Flux and Mitochondrial DNA Dynamics During Circadian-Aligned Ramadan Dry Fasting Versus 16:8 Time-Restricted Feeding: A Four-Phase Translational Research Programme","MITO FAST","Inclusion Criteria:\n\n* Common Criteria (Both Arms):\n* Age 18-50 years\n* BMI 18.5-30.0 kg\u002Fm²\\[cite: 1\\]\n* Non-smoker for at least 12 months\\[cite: 1\\]\n* No chronic disease requiring regular prescription medication\\[cite: 1\\]\n* Stable sleep-wake schedule (self-reported sleep midpoint deviation less than 90 minutes)\\[cite: 1\\]\n* Willing and able to attend all five study visits at the specified times\\[cite: 1\\]\n\nArm A Specific (Ramadan Dry Fasting):\n\n\\- Documented religious commitment and intention to complete all 30 days of Ramadan 2027 fasting\\[cite: 1\\]\n\nArm B Specific (16:8 TRF Control):\n\n* Confirmed practice of 16:8 TRF (fasting window 16 hours or more per day) throughout the study period\\[cite: 1\\]\n* Not observing Ramadan dry fasting during the study period\\[cite: 1\\]\n\nExclusion Criteria:\n\n* Established type 1 or type 2 diabetes (HbA1c 6.5% or higher or pharmacological antidiabetic therapy)\\[cite: 1\\]\n* Severe gastrointestinal, cardiovascular, hepatic, or renal disease (eGFR less than 60 mL\u002Fmin\u002F1.73m²)\\[cite: 1\\]\n* Active cancer or chemotherapy within five years\\[cite: 1\\]\n* Use of metformin, statins, or exogenous antioxidant supplements (vitamins C, E, NAC, CoQ10) within 30 days of screening\\[cite: 1\\]\n* Pregnancy, lactation, or planned conception during the study period\\[cite: 1\\]\n* Participation in another clinical study within three months\\[cite: 1\\]\n* Inability to provide written informed consent in Arabic or English\\[cite: 1\\]",true,"ALL","18 Years","50 Years",{"count":91,"type":92},120,"ESTIMATED","OBSERVATIONAL","This study investigates how different types of fasting affect cell health, specifically focusing on how mitochondria (the energy-producing parts of cells) are cleared and renewed. While standard intermittent fasting allows water intake, Ramadan dry fasting involves total restriction of both food and fluids from dawn to sunset.\n\nResearchers want to see if the combined effects of fluid restriction and natural daily body rhythms during Ramadan trigger a stronger cellular cleanup process (called mitophagy) compared to standard 16:8 water-permitted fasting. The study will look at how these fasting habits change blood markers related to mitochondrial DNA and metabolic health across a 30-day period. This research will help determine if dry fasting offers distinct biological benefits for cellular renewal.",[96,97,98,99,100],"Mitochondrial Degradation","Autophagy","Fasting","Healthy Volunteers","Metabolic Adaptation",[102,103,104,105,106,107,108],"Ramadan fasting","Intermittent dry fasting","Time-restricted feeding","Mitochondrial DNA","Mitophagy","PBMC","PINK1-Parkin","NOT_YET_RECRUITING","2026-06-09",{"date":112,"type":113},"2026-06-11","ACTUAL",{"date":115,"type":92},"2027-01-15",{"date":117,"type":92},"2027-04-30",{"name":5,"class":6},1]