[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"health-study-detail:100657067":3},{"organization":4,"outcomesModule":7,"designInfo":31,"detailedDescription":38,"studyPopulation":30,"armGroups":39,"interventions":46,"overallOfficials":52,"centralContacts":56,"locations":62,"responsibleParty":80,"collaborators":30,"id":84,"slug":85,"hasResults":86,"nctId":87,"briefTitle":88,"officialTitle":89,"acronym":90,"eligibilityCriteria":91,"healthyVolunteers":92,"sex":93,"minAge":94,"maxAge":95,"enrollmentInfo":96,"targetDuration":30,"studyType":99,"phases":100,"briefSummary":101,"conditions":102,"keywords":105,"overallStatus":114,"whyStopped":30,"lastUpdateSubmitDate":115,"lastUpdatePostDateStruct":116,"startDateStruct":119,"completionDateStruct":121,"leadSponsor":123,"locationsCount":124},{"fullName":5,"class":6},"University of Thi-Qar","OTHER",{"primaryOutcomes":8,"secondaryOutcomes":16,"otherOutcomes":30},[9,13],{"measure":10,"description":11,"timeFrame":12},"Change in Adenosine Triphosphate (ATP) Concentration","Researchers measure ATP levels in blood cells using a specific assay kit to evaluate cellular energy production.","Baseline and immediately after the 10-to-20-minute light therapy session",{"measure":14,"description":15,"timeFrame":12},"Change in Cytochrome c Oxidase Activity","Researchers assess the specific activity of cytochrome c oxidase in blood samples via a biochemical assay to determine mitochondrial function.",[17,20,24,27],{"measure":18,"description":19,"timeFrame":12},"Change in Malondialdehyde (MDA) Levels","Researchers quantify MDA concentration in the collected blood samples to assess oxidative stress.",{"measure":21,"description":22,"timeFrame":23},"Change in Lactate and Pyruvate Concentrations","Researchers determine the concentrations of lactate and pyruvate in blood cells to analyze metabolic shifts.","Baseline and immediately after the 10 to 20-minute light therapy session",{"measure":25,"description":26,"timeFrame":12},"Change in Glutathione (GSH) Levels","Researchers measure glutathione concentration in the blood samples to evaluate antioxidant capacity.",{"measure":28,"description":29,"timeFrame":12},"Change in Blood Glucose Levels","Researchers measure blood glucose concentrations in the collected blood samples to evaluate glycemic changes following the light therapy session.",null,{"allocation":32,"interventionModel":33,"interventionModelDescription":34,"primaryPurpose":35,"observationalModel":30,"timePerspective":30,"maskingInfo":36},"NA","SINGLE_GROUP","The study assigns all participants, encompassing Type 1 Diabetes patients and healthy volunteers, to a single group receiving the identical photobiomodulation intervention. Researchers expose the participants to red and near-infrared light for 10 to 20 minutes. The resulting biochemical data is stratified during the statistical analysis phase to evaluate and compare the responses between the two cohorts.","BASIC_SCIENCE",{"masking":37,"maskingDescription":30,"whoMasked":30},"NONE","This study evaluates the metabolic effects of photobiomodulation on peripheral blood mononuclear cells in patients with Type 1 diabetes and healthy volunteers. The intervention applies red (660 nm) and near-infrared (850 nm) light to stimulate cytochrome c oxidase within the mitochondrial electron transport chain. This photon absorption increases cellular respiration and alters oxidative stress markers.\n\nResearchers collect baseline venous blood samples from all participants to establish initial biochemical parameters. The clinical team isolates peripheral blood mononuclear cells using density gradient centrifugation. Following baseline collection, participants receive a single 10-to-20-minute light therapy session on the upper arm using a flexible light pad. Researchers collect a second venous blood sample immediately after the light exposure.\n\nThe laboratory analysis quantifies specific mitochondrial and metabolic variables in the cell lysates before and after the intervention. The primary metrics are adenosine triphosphate (ATP) concentration and cytochrome c oxidase specific activity. Secondary measures include malondialdehyde and glutathione to assess lipid peroxidation and antioxidant capacity. The research team also measures lactate and pyruvate levels to evaluate shifts in cellular glycolysis, alongside standard fasting plasma glucose monitoring. The comparative analysis evaluates differences in metabolic response between the diabetic cohort and the healthy matched controls.",[40],{"label":41,"type":42,"description":43,"interventionNames":44},"Photobiomodulation Intervention","EXPERIMENTAL","Researchers apply the photobiomodulation device to the arm of every enrolled participant, encompassing both the healthy volunteers and the patients diagnosed with Type 1 diabetes, for a strictly timed session lasting between 10 and 20 minutes.",[45],"Device: Red and Near-Infrared Light Therapy Pad",[47],{"type":48,"name":49,"description":50,"armGroupLabels":51,"otherNames":30},"DEVICE","Red and Near-Infrared Light Therapy Pad","Participants receive a single photobiomodulation session using a flexible light therapy pad. The device delivers red light at 660 nm and near-infrared light at 850 nm with an operating voltage of 5V and a working current of 1.7A. The pad is placed in direct contact with the upper arm of each participant for a timed duration of 10 to 20 minutes. Researchers evaluate cellular responses before and immediately after this single application.",[41],[53],{"name":54,"affiliation":5,"role":55},"Hattf B. Farhood, PhD","STUDY_DIRECTOR",[57],{"name":58,"role":59,"phone":60,"phoneExt":30,"email":61},"Joudi k. Shaker, MSc","CONTACT","+964-780-630-0739","j.shaker@utq.edu.iq",[63],{"facility":64,"status":30,"city":65,"state":66,"zip":67,"country":68,"countryCode":69,"cosmosGeoPoint":70,"geoPoint":75,"contacts":76},"Al Nasiriya Teaching Hospital","Nasiriyah","TQ","64001","Iraq","IQ",{"type":71,"coordinates":72},"Point",[73,74],46.25726,31.05799,{"lat":74,"lon":73},[77],{"name":78,"role":59,"phone":79,"phoneExt":30,"email":61},"Joudi Shaker, MSc","+9647806300739",{"type":81,"investigatorFullName":82,"investigatorTitle":83,"investigatorAffiliation":5,"oldNameTitle":30,"oldOrganization":30},"PRINCIPAL_INVESTIGATOR","Joudi Shaker","MSc Researcher","100657067","the-effect-of-light-therapy-on-cellular-energy-and-oxidative-stress-in-healthy-adults-and-type-1-diabetic-patients-100657067",false,"NCT07833865","The Effect of Light Therapy on Cellular Energy and Oxidative Stress in Healthy Adults and Type 1 Diabetic Patients","Biochemical Study of Metabolic Regulation and Extraction of Mitochondrial Biomarkers in Type 1 Diabetes Mellitus in Thi-Qar Governorate, Iraq","PBM-T1D","Inclusion Criteria:\n\n* Age 12 to 40 years.\n* Confirmed diagnosis of Type 1 Diabetes Mellitus based on American Diabetes Association criteria (patient group).\n* Insulin dependence (patient group).\n* Low serum C-peptide levels (patient group).\n* Fasting plasma glucose concentration \\\u003C100 mg\u002FdL (healthy control group).\n* Glycated haemoglobin (HbA1c) \\\u003C5.7% (healthy control group).\n* Diabetes duration of at least 1 year (patient group).\n\nExclusion Criteria:\n\n* Diabetic ketoacidosis within the preceding month.\n* High-sensitivity C-reactive protein (hs-CRP) \\>10 mg\u002FL or active infection.\n* Renal insufficiency.\n* Liver disease.\n* Pregnancy.\n* Smoking.\n* Use of antioxidant supplements, vitamin C, or vitamin E within the preceding month.\n* Autoimmune diseases other than Type 1 Diabetes Mellitus.\n* Haematological disorders.",true,"ALL","12 Years","40 Years",{"count":97,"type":98},30,"ESTIMATED","INTERVENTIONAL",[32],"The goal of this clinical trial is to evaluate the effects of low-level light therapy on cellular energy and oxidative stress in healthy adults and patients with Type 1 diabetes. The main questions it aims to answer are:\n\nDoes light therapy change adenosine triphosphate (ATP) levels and cytochrome c oxidase activity in blood cells? How does the intervention affect oxidative stress markers, specifically glutathione and malondialdehyde concentrations? Researchers will compare blood samples collected before the light therapy to those collected afterward to see if the intervention alters mitochondrial function. Researchers will also compare the responses of healthy volunteers to those of diabetic patients.\n\nParticipants will:\n\nProvide a baseline blood sample. Receive red and near-infrared light exposure (660 nm and 850 nm) on their arms for 10 to 20 minutes using a therapy pad.\n\nProvide a final blood sample immediately after the light exposure.",[103,104],"Healthy","Diabetes Mellitus Type 1",[106,107,108,109,110,111,112,113],"Photobiomodulation","Malondialdehyde","Glutathione","Mitochondria","Cytochrome c Oxidase","Adenosine Triphosphate","Oxidative Stress","Low-Level Light Therapy","NOT_YET_RECRUITING","2026-10-09",{"date":117,"type":118},"2026-10-13","ACTUAL",{"date":120,"type":98},"2026-10-01",{"date":122,"type":98},"2027-01-15",{"name":5,"class":6},1]