The purpose of this study is to assess the amino acid oxidation dose-response relationship of ingested whole-food protein in healthy adults. In a randomized, crossover design, 20 young, healthy adult males and females aged 18-35 years, will consume a dinner consisting of 0.32, 0.74, and 1.32 g protein･kg BM-1 (corresponding to 1, 2, and 4 minced beef patties, respectively) during 3 separate experimental trials.The main study endpoint is the amino acid oxidation of ingested protein over a 24-h period.
Aged between 18-35 years
BMI between 18.5-30 kg/m2
Healthy, recreationally active (exercise at least once per two weeks and a maximum of four days per week)
No physical limitations (i.e., able to perform all activities associated with daily living independently)
Smoking
Vegan, vegetarian, or does not consume beef protein
Diagnosed musculoskeletal disorders
Use of any medications known to affect protein metabolism (i.e., corticosteroids, non-steroidal anti-inflammatories, or prescribed acne medications)
Crossover
Food proteins
Dietary supplementDinner meal consisting of minced beef protein.
Amino acid oxidation of ingested protein
Amino acid oxidation; compared between interventions to determine the dose-response relationship of amino acid oxidation to ingested whole-food protein.
Plasma amino acid response of ingested protein
Plasma amino acid response; comparison between interventions to determine the plasma amino acid response to different amounts of ingested whole-food protein.
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