About this trial
The aims of the study are:
1. To examine skeletal muscle and blood metabolites during an official basketball game and to relate those to skeletal muscle performance during and after the game. 2. To investigate whether skeletal muscle and blood metabolites are related to game-induced fatigue. 3. To relate field activity during a basketball game with the responses of skeletal muscle and blood metabolites. 4. To investigate how blood lactate reflects muscle lactate concentration during a basketball game. 5. To determine whether temporary fatigue develops during a basketball game. game.
Eligibility criteria
This trial accepts healthy volunteersQualifiers
Health status: were free of recent musculoskeletal injuries and/or illnesses (≥ 8 months before the study).
Not consuming ergogenic supplements/medications (≥ 6 months before the study)
Not smokers
Disqualifiers
Completion of the pre-determined participation time during the experimental game.
Completion of all field measurements.
Completion of all pre-determined sampling points for biological tissue.
Trial design
Single group
Treatments tested in this trial
BASKETBALL GAME
Other interventionParticipants will arrive 1.5 hours pre-game for initial vastus lateralis muscle biopsies and blood sampling under local anesthesia (20 mg/ml xylocaine without adrenalin). They will be fitted with IMU sensors and heart rate monitors. Volunteers are randomly assigned to teams(6 teams) for a full 40-minute game. During the match, physiological measurements will be performed at fixed times (pre, at half-time, and post-match) and after intense exercise periods (HR\>90% max, high-speed runs\>18km/h) within the four 10-min periods. Three groups provide samples: Group 1 (n=15): rest, half-time, end-game. Group 2 (n=8): rest, post-intense period in 1st/3rd periods. Group 3 (n=7): rest, post-intense period in 2nd/4th periods. Ten players serve as substitutes. Games will start at 17.00 - 18 p.m. to avoid diurnal variations and will be held under normal environmental conditions. A standardized 30-min warm-up period will be performed before each game.
Treatment groups
Trial outcomes
Primary outcomes
Measurement of countermovement jump height
Will be measured using a force platform system
Bilateral strength peak force and rate of force development on mid-thigh pull
Will be measured using a dynamometer.
Measurement of maximal sprinting speed over 5 meters
5-m sprinting speed will be assessed on the basketball court using infrared photocells
Measurement of maximal sprinting speed over 20 meters
20-m sprinting speed will be assessed on the basketball court using infrared photocells
Secondary outcomes
Body mass
Body mass will be measured using a beam balance
Body height
Body height will be measured using a beam balance with a stadiometer
Body mass index (BMI)
BMI will be calculated using the Quetelet's equation
Body fat (BF)
Body fat (%) will be assessed by whole-body dual-energy X-ray absorptiometry (DXA)
Sponsors and contacts
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University of Thessaly
Lead sponsor
University of Southern Denmark
Collaborator
Democritus University of Thrace
Collaborator