Application of Nanopore Adaptive Sequencing
ConditionLiver Transplant Infection
Trial statusNot yet recruiting
Trial phaseNot listed
Trial typeObservational
Biological sexAll
AgeNot listed
SponsorShenzhen Third People's Hospital
About this trial
Infection post liver transplantation is an important factor in the death in patients. The traditional method of diagnosing infection post liver transplantation is laboratory tests. But the sensitivity and specificity of blood tests is poor. Next-generation sequencing (NGS) has greater detection rate for mycobacterium tuberculosis, anaerobes and fungi and greater sensitivity compare with blood tests. However use of NGS is limited because of the short read-length. Oxford nanopore adaptive sequencing (NAS) method is the Third Revolution in Sequencing Technology. For each 1 Gbp of data, NAS sequencing detected 45 times more microbiome sequences than Nanopore standard sequencing and 2.5 times more than Illumina sequencing. The purpose of this study is to compare NAS with NGS and laboratory tests for the diagnostic rate of infection post liver transplantation.
Eligibility criteria
This trial does not accept healthy volunteersQualifiers
Accept liver transplantation
Signing informed consent voluntarily
Possessing ability to comprehend material information
Participating this study voluntarily
Disqualifiers
Participated another study
Graft loss
Have undergone a multi-organ transplantion or have had a previous organ transplantation
Patient died
Trial population
Participants will be recruited randomly and if they violate the protocol or occur clinical adverse events, the investigators will ask them to withdraw from this study.
Trial design
Design model
Case-only
Time perspective
Prospective
Treatments tested in this trial
NAS, NGS and Laboratory tests
Diagnostic testUsing three diffrent detection methods and comparing their efficiency
Treatment groups
100 Participants
are divided into 3 treatment groupsGroup A: NAS group1 intervention
Group B: NGS group1 intervention
Group C: Lab Tests group1 intervention
Trial outcomes
Primary outcomes
1
Diagnosed infection post liver transplantation
Any pathogens are detected in alveolar lavage fluid, peritoneal drainage or blood before patients discharge from hospital, an average of 1 months
Time frame
Post liver transplantation before patients discharge from hospital, an average of 1 months.Sponsors and contacts
Click on the lead sponsor to view all of their trials.
Source ClinicalTrials.gov
This trial is not recruiting at the moment. You can still explore other options: