Acute bronchiolitis is a common disease in children under the age of two, caused mainly by the respiratory syncytial virus (RSV). Furthermore, given the same medical history, it is still very difficult to predict the course and severity of the infection at the onset of symptoms, Some studies have highlighted the importance of the microbiota (intestinal, oral or nasopharyngeal) and of the immune response to RSV in children, We will include 80 children under 2 years old with hospitalized bronchiolitis and non-hospitalized bronchiolitis. Oral, nasal and stool samples will be taken to study the various microbiota in search of dysbiosis. A capillary blood sample will be taken for immune studies.
Infants <12 months
With bronchiolitis during RSV epidemic season
No chronic illness
No bronchiolitis medical history
Chronic respiratory illness
Medical history of bronchiolitis or newborn asthma
Treatment with immunosuppressants
Patient with no social security affiliation
Bronchiolitis in patient under 12 months old
Cohort
Prospective
Sampling
Other interventionSingle-time sampling of respiratory virus-infected children
Dysbiosis
comparison of the quantitative and qualitative composition of bacteria (alpha diversity, beta diversity, Shannon and Simpson index) in the digestive and nasopharyngeal microbiota
Measurement of innate and adaptive responses by quantification of cytokines and chemokines in plasma
Inflammatory/regulatory cytokines (IL1-\>17, IL-10, TGF-b, EGF, FGF-2, IFN-alpha, IFN-gamma, IFN-beta, MCRP-1 MCP-3, TNF-alpha...), cytokines of adaptative response (Th1, Th17, Th2 and/or Treg), markers of inflammatory response (interferon, TNFa, …), quantified by Luminex
Identifying the mRNA profile in blood samples and nasal swabs
mRNA will be identified by using RNA-Seq (Illumina)
Sequencing viral strains for mutation
Typing of respiratory syncytial virus-A (RSV-A) and B (RSV-B) and sequencing of strains (Amplicons)
Comparison of respiratory syncytial virus (RSV) antibodies levels on newborn screening specimen and on capillary swab during infection
Levels of RSV antibodies will be measured and compared between newborn screening specimen and capillary swab during infection
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Assistance Publique - Hôpitaux de Paris
Lead sponsor
Infectious Diseases Models for Innovative Therapies center
Collaborator
