About this trial
This study aims to investigate acute changes in serum ferritin, folate, vitamin B12, and parathyroid hormone (PTH) levels following aerobic exercise in professional soccer players. Twenty professional male soccer players will voluntarily participate in the study. Participants will complete a progressive 20-meter shuttle run test. Venous blood samples will be collected immediately before and after the exercise protocol. Pre- to post-exercise changes in each blood parameter will be analyzed to determine the acute physiological responses to aerobic exercise in professional soccer players.
Eligibility criteria
This trial accepts healthy volunteersQualifiers
Professional soccer players.
Willing and able to participate in the study procedures.
Provision of informed consent.
Disqualifiers
Inability to complete the aerobic exercise protocol.
Trial design
Single group
Treatments tested in this trial
20-Meter Shuttle Run Test
BehavioralParticipants will perform a progressive 20-meter shuttle run test by running continuously between two lines positioned 20 meters apart in time with prerecorded audio signals. Running speed will progressively increase as the interval between the audio signals decreases. The test will end when the participant fails to reach the designated line before the signal on two consecutive occasions.
Treatment groups
Trial outcomes
Primary outcomes
Change in Serum Ferritin Concentration From Pre-Exercise to Immediately Post-Exercise
Venous blood samples will be collected immediately before and immediately after the 20-meter shuttle run test. Serum ferritin concentration will be measured in ng/mL. For each participant, the change will be calculated by subtracting the pre-exercise value from the post-exercise value. The group-level outcome will be summarized using the mean change and standard deviation.
Change in Serum Folate Concentration From Pre-Exercise to Immediately Post-Exercise
Venous blood samples will be collected immediately before and immediately after the 20-meter shuttle run test. Serum folate concentration will be measured in ng/mL. For each participant, the change will be calculated by subtracting the pre-exercise value from the post-exercise value. The group-level outcome will be summarized using the mean change and standard deviation.
Change in Serum Vitamin B12 Concentration From Pre-Exercise to Immediately Post-Exercise
Venous blood samples will be collected immediately before and immediately after the 20-meter shuttle run test. Serum vitamin B12 concentration will be measured in pg/mL. For each participant, the change will be calculated by subtracting the pre-exercise value from the post-exercise value. The group-level outcome will be summarized using the mean change and standard deviation.
Change in Serum Parathyroid Hormone Concentration From Pre-Exercise to Immediately Post-Exercise
Venous blood samples will be collected immediately before and immediately after the 20-meter shuttle run test. Serum parathyroid hormone (PTH) concentration will be measured in pg/mL. For each participant, the change will be calculated by subtracting the pre-exercise value from the post-exercise value. The group-level outcome will be summarized using the mean change and standard deviation.
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