Boron Levels, Oxidative Stress, and Inflammation in Periodontitis
ConditionPeriodontitis
Trial statusRecruiting
Trial phaseNot listed
Trial typeObservational
Biological sexAll
Age18-65
SponsorMarmara University
About this trial
This cross-sectional observational study aims to evaluate the relationship between boron levels and oxidative stress, antioxidant defense, and inflammatory markers in individuals with periodontitis. The study will include systemically healthy, non-smoking individuals divided into two groups: periodontally healthy controls and patients with Stage III-IV periodontitis. Boron levels and selected oxidative stress, antioxidant, and inflammatory biomarkers will be evaluated in saliva and gingival tissue samples. The findings will be compared between the groups to investigate the potential relationship between boron levels and periodontal inflammation and oxidative stress.
Eligibility criteria
This trial accepts healthy volunteersQualifiers
Volunteering to participate in the study and signing the informed consent form
Being between 18 and 65 years of age
Being systemically healthy
No antibiotic use within the last 3 months
Disqualifiers
Incomplete or unreliable clinical measurements
Insufficient saliva or gingival tissue sample for biochemical analyses
Regular use of boron-containing oral care products within the last 3 months
Regular consumption of boron-rich supplements or mineral waters
Trial population
The study population will consist of 60 systemically healthy individuals aged 18-65 years attending the Department of Periodontology, Faculty of Dentistry, Marmara University. Participants will be divided into two groups: 30 individuals with Stage III-IV periodontitis with an indication for periodontal flap surgery and 30 periodontally healthy individuals with an indication for crown-lengthening surgery.
Trial design
Design model
Cohort
Time perspective
Cross-sectional
Treatments tested in this trial
Biochemical Assessment of Saliva and Gingival Tissue
Other interventionUnstimulated saliva and gingival tissue samples will be collected from participants. Gingival tissue samples will be obtained during clinically indicated periodontal surgical procedures. The samples will be biochemically analyzed to determine boron levels and markers of oxidative stress, antioxidant defense, and inflammation. No experimental treatment or therapeutic intervention will be administered as part of the study.
Treatment groups
60 Participants
are divided into 2 treatment groupsGroup A: Periodontitis Group1 intervention
Group B: Periodontally Healthy Group1 intervention
Trial outcomes
Primary outcomes
1
Boron Levels in Saliva and Gingival Tissue
Boron levels will be measured in unstimulated saliva and gingival tissue samples obtained from participants in the periodontitis and periodontally healthy groups. Salivary boron levels will be expressed as µg/mL and gingival tissue boron levels as µg/g.
Time frame
At the time of the clinically indicated periodontal surgical procedureSecondary outcomes
1
Oxidative Stress Markers
Oxidative stress will be assessed by measuring reactive oxygen species (ROS), total oxidant status (TOS), and malondialdehyde (MDA) levels in samples obtained from the periodontitis and periodontally healthy groups.
Time frame
At the time of the clinically indicated periodontal surgical procedure2
Antioxidant Defense Markers
Antioxidant defense will be assessed by measuring total antioxidant status (TAS), glutathione (GSH), superoxide dismutase (SOD), and catalase (CAT) levels in samples obtained from the periodontitis and periodontally healthy groups.
Time frame
At the time of the clinically indicated periodontal surgical procedure3
Inflammatory Marker
Inflammation will be assessed by measuring interleukin-1 beta (IL-1β) levels in samples obtained from the periodontitis and periodontally healthy groups.
Time frame
At the time of the clinically indicated periodontal surgical procedureSponsors and contacts
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Source ClinicalTrials.gov