C-Protein in Fatigue and Aging
Trial statusRecruiting
Trial phaseNot applicable
Trial typeInterventional
Biological sexAll
Age18-80
SponsorUniversity of Oregon
About this trial
This study is investigating changes to the proteins in skeletal muscle that contribute to reduced muscle size and muscle function that occurs with aging.
Eligibility criteria
This trial accepts healthy volunteersQualifiers
Adults between the ages 18-35 or 65-80 years
Healthy by self-report
Willing to participate in all aspects of the study design including muscle biopsy, unilateral exercise, and physical activity monitoring
Fluent in English (due to lack of translation services, it is not practical to conduct the study using a language other than English).
Disqualifiers
Orthopaedic limitation (severe knee osteoarthritis, prior joint replacement, etc.).
Volunteer has dementia or related mental issues that potentially put the subject at risk as determined by prior diagnosis.
Volunteer has known untreated endocrine disease (hypo/hyper thyroidism, Addison's Disease or Cushing's syndrome, etc.)
Volunteer has untreated/poorly-controlled hypertension (stage 2, per American College of Cardiology [>140/90 mmHg]).
Trial design
Design model
Single group
Treatments tested in this trial
Fatiguing Exercise
BehavioralVolunteers will perform knee extension exercise of the dominant limb while seated in a chair designed for exercise of the knee extensor muscle group. The chair will be equipped with a lever arm, aligned with the axis of rotation of the knee and fixed to the distal shank, 2 centimeters proximal to the ankle joint. The lever arm will be instrumented to provide resistance and measure velocity. The volunteers will perform 3-5 maximum voluntary contractions (MVC) to determine peak torque. Once established, volunteers will rest for 5 minutes before initiating the fatigue protocol, whereby they will perform repeated MVC of the dominant limb against resistance equal to or less than 50% of MVC until range of motion cannot be maintained. Because the goal of this exercise is to uniformly fatigue the quadriceps muscle group, this exercise will be performed continuously. It is expected to take between 1 and 3 minutes to achieve the desired level of fatigue.
Treatment groups
24 Participants
are divided into 1 treatment groupGroup A: FatigueExperimental treatment 1 intervention
Trial outcomes
Primary outcomes
1
Phosphorylation of myosin binding protein c (MyBP-C)
Muscle tissue is immediately frozen after biopsy. Liquid chromatography and high resolution mass spectrometry will be used to do a full analysis of the phosphorylation sites on MyBP-C isolated from the biopsied muscle tissue.
Time frame
One week after the participant undergoes the biopsies.Secondary outcomes
1
Muscle fiber Force
Ex vivo assessment of Force (mN) will be performed on muscle fibers and bundles isolated from fatigued and non-fatigued muscle biopsies.
Time frame
Within three weeks of the intervention and biopsies.2
Muscle fiber Tension
Ex vivo assessment of Tension (kPa) will be performed on muscle fibers and bundles isolated from fatigued and non-fatigued muscle biopsies.
Time frame
Within three weeks of the intervention and biopsies.3
Muscle fiber Velocity
Ex vivo assessment of Velocity (Lo/s) will be performed on muscle fibers and bundles isolated from fatigued and non-fatigued muscle biopsies.
Time frame
Within three weeks of the intervention and biopsies.4
Muscle fiber Power
Ex vivo assessment of Power (w/L) will be performed on muscle fibers and bundles isolated from fatigued and non-fatigued muscle biopsies.
Time frame
Within three weeks of the intervention and biopsies.Sponsors and contacts
Click on the lead sponsor to view all of their trials.
University of Oregon
Lead sponsor
Wu Tsai Human Performance Alliance
Collaborator
National Institutes of Health (NIH)
Collaborator
Oregon Health and Science University
Collaborator
Washington State University
Collaborator
National Institute on Aging (NIA)
Collaborator
Source ClinicalTrials.gov