The role of the intestinal microbiota on health is now well recognised, and diet is one of the factors influencing the maintenance of a healthy intestinal microbiota. More specifically, the consumption of fruit, vegetables and cereals is associated with good health and, in particular, with an increase in the relative abundance of bacterial groups described as beneficial. To date, a large number of bacterial species in the intestinal microbiota have yet to be isolated and cultivated, which limits the characterisation of their potential health benefits. In order to isolate and cultivate these intestinal bacteria, it is particularly relevant to recruit individuals with a high-fibre diet.
Healthy adult subjects ;
Age between 20 and 50 (limits included) ;
BMI between 18.5 and 25 kg/m2 (excluding limits) ;
Consumption of more than 5 portions of fruit and vegetables a day;
Antibiotics and/or transit modulators taken in the 3 months prior to sampling;
Known pathologies;
Current treatment;
History of digestive surgery having an impact on the microbiota, deemed incompatible with the study (bariatric surgery);
General population residing in Clermont-Ferrand (France)
Cohort
Prospective
Not listed
Isolation and culture of one isolate from each target species
Successful isolation and culture of at least one isolate from each target species previously identified through metagenomic data analyses.
metagenomic profiles in relation to dietary profiles
To obtain metagenomic profiles using shotgun sequencing of the gut microbiota (bacterial species), in relation to dietary profiles
growth capacity of the isolates
To characterize the growth (growth monitoring by optical density) of the isolates of interest.
Functional potential of the isolates
To characterize the genomic features of the isolates of interest (functional potential through genome annotation).
Phenotypic caracterization of the isolates
To characterize phenotypically the isolates of interest by API gallery.
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Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
Lead sponsor
Centre de Recherche en Nutrition Humaine d'Auvergne
Collaborator
