Demodex Mites in Acne Patients and Herbal Extract Susceptibility
Trial statusNot yet recruiting
Trial phaseNot listed
Trial typeObservational
Biological sexAll
Age18-45
SponsorAssiut University
About this trial
This case-control study aims to detect the presence and density of Demodex mites in patients with acne, identify the species using molecular methods, and evaluate the in vitro survival time of live mites exposed to medicinal herbal extracts.
Eligibility criteria
This trial accepts healthy volunteersQualifiers
- Age 18 to 45 years
Clinically diagnosed acne vulgaris of any severity (cases)
Age- and sex-matched healthy individuals without dermatosis or pruritus (controls)
Written informed consent
Disqualifiers
- Use of topical anti-acne or acaricidal treatments within the last 4 weeks
Use of systemic antibiotics or systemic retinoids within the last 3 months
Trial population
Adult patients with clinically diagnosed acne vulgaris attending the dermatology outpatient clinic at Assiut University Hospital, and age- and sex-matched healthy controls.
Trial design
Design model
Case-control
Time perspective
Cross-sectional
Treatments tested in this trial
Not listed
Trial groups
80 Participants
are grouped into 2 trial groupsGroup A: Patients with Acne
Group B: Healthy Controls
Trial outcomes
Primary outcomes
1
Prevalence of Demodex infestation
Proportion of participants with Demodex mites detected by standardized skin surface biopsy.
Time frame
Baseline2
Mean Demodex density
Mean number of Demodex mites per cm² of skin surface.
Time frame
Baseline3
Mean in vitro survival time of Demodex mites
Mean survival time in minutes of live Demodex mites exposed to the medicinal herbal extracts.
Time frame
BaselineSecondary outcomes
1
Distribution of Demodex species
Frequency and percentage of Demodex folliculorum and Demodex brevis among positive cases identified by PCR.
Time frame
Baseline2
Mean mite density according to acne severity
Mean Demodex density (mites/cm²) in mild, moderate, and severe acne groups according to the Global Acne Grading System.
Time frame
Baseline3
Mean survival time at 5% herbal extract concentration
Mean survival time in minutes of Demodex mites exposed to 5% concentration of the herbal extract
Time frame
Baseline4
Mean survival time at 10% herbal extract concentration
Mean survival time in minutes of Demodex mites exposed to 10% concentration of the herbal extract.
Time frame
BaselineLocations
Sponsors and contacts
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Source ClinicalTrials.gov
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