Health TrialsbyHealthPanel

Diet Synergy in Muscle Protein Synthesis

ConditionsMuscle Protein Synthetic Response to ProteinMuscle Protein SynthesisMuscle HealthProtein MetabolismRecreational ActivitiesIsotope Labeling
Trial statusRecruiting
Trial phaseNot applicable
Trial typeInterventional
Biological sexAll
Age19-40
SponsorUniversity of Illinois at Urbana-Champaign

About this trial

In the present semi-crossover randomized design, 15 recreationally active young adults (19-40 y) will receive stable isotope tracer infusions and perform a single bout of resistance exercise. Immediately after exercise, participants will ingest either 1 medium russet potato with skins and 2 ounce-equivalent lean ground beef combination (274 kcals, 19.3 g protein, 5 g fat, 37 g carbohydrates, and 4 g fiber), 2 slices of white bread and beef combination (262 kcals, 19.8 g protein, 6.8 g fat, 28 g carbohydrates, and 1.8 g fiber) or 2 ounce-equivalent beef (93% lean meat/7% fat; 110 kcals, 14.8 g protein, and 5 g fat). Repeated blood and muscle biopsies will be collected to determine whole body leucine kinetics, amino acid concentrations, anabolic signaling and myofibrillar protein synthesis rates during the trials.

Eligibility criteria

This trial accepts healthy volunteers

Qualifiers

Age 19-40 yrs

Pre-menopausal

Recreationally-active

Weight stable for prior 6 months

Disqualifiers

Age outside of range (20 - 35 yrs)

Pregnancy

Irregular menstrual cycles (i.e., ~21 day or ~35 day cycle)

Participation in previous research using L-[1-13C] leucine and L-[ring-2H5] phenylalanine

Trial design

Design model

Crossover

Treatments tested in this trial

  • Resistance Exercise

    Behavioral

    Participants will perform leg press and leg extension immediately prior to ingestion of either potato-beef combination, bread-beef combination, or beef alone.

  • Potato and Beef

    Dietary supplement

    Participants will ingest baked russet potato and cooked ground beef immediately after resistance exercise.

  • Bread and Beef

    Dietary supplement

    Participants will ingest white bread and cooked ground beef immediately after resistance exercise.

  • Beef

    Dietary supplement

    Participants will ingest cooked ground beef immediately after resistance exercise.

Treatment groups

15 Participants
are divided into 3 treatment groups
Group A: Potato and Beef (PB)Experimental treatment 2 interventions
Group B: Bread and Beef (BB)Experimental treatment 2 interventions
Group C: Beef (B)Experimental treatment 2 interventions

Trial outcomes

Primary outcomes

1

Myofibrillar protein fractional synthesis rate

Myofibrillar protein synthesis rates will be assessed during the postabsorptive and postprandial periods of experimental primed-constant stable isotope infusions.

Time frame
Baseline muscle biopsy (-150 minutes), Postabsorptive muscle biopsy (-30 minutes), and Postprandial muscle biopsy (300 minutes)
2

Whole body leucine kinetics

Whole body leucine kinetics will be determined via breath sample collection during the postabsorptive and postprandial periods of experimental primed-constant stable isotope infusions and analyzed via LC/MS/MS.

Time frame
Breath sample collection time points: -210, -150, -90, -60, -30, 0, 15, 30, 45, 60, 90, 120, 150, 180, 210, 240, and 300 minutes.

Secondary outcomes

1

Activation of anabolic signaling targets

Phosphorylation of mTOR-dependent anabolic signaling targets (i.e., 4E-BP1, S6K1, and rps) will be assessed via Western Blotting (phosphorylated-to-total ratio).

Time frame
Baseline muscle biopsy (-150 minutes), Postabsorptive muscle biopsy (-30 minutes), and Postprandial muscle biopsy (300 minutes)

Other outcomes

Sponsors and contacts

Click on the lead sponsor to view all of their trials.

University of Illinois at Urbana-Champaign

Lead sponsor

Source ClinicalTrials.gov
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