Health TrialsbyHealthPanel

DNA Methylation and Vascular Function

ConditionsObesityVascular Dysfunction
Trial statusRecruiting
Trial phaseNot applicable
Trial typeInterventional
Biological sexAll
Age18-50
SponsorUniversity of Illinois at Chicago

About this trial

The main objective is to examine DNA hypomethylation as an underlying mechanism for the increased production of inflammatory cytokines and the impaired vascular function in obese individuals and as a potential target for nonpharmacological preventive/therapeutic interventions such as aerobic exercise.

Eligibility criteria

This trial accepts healthy volunteers

Qualifiers

BMI ≥ 35 kg/m2

Between ages 18-50 years

Not pregnant

Approved for a bariatric surgery

Disqualifiers

To avoid confounding from other inflammatory conditions individuals with current cancer, heart, kidney or liver disease, gallbladder disease or acute or chronic inflammatory diseases (including rheumatoid arthritis, lupus and other autoimmune diseases and genetic diseases) will be excluded

Pregnant women will be excluded, as they will not be eligible for bariatric surgery

Current smokers

Currently abusing alcohol or drugs

Trial design

Design model

Parallel

Treatments tested in this trial

  • Exercise training

    Other intervention

    Twelve weeks of aerobic exercise training

Treatment groups

80 Participants
are divided into 2 treatment groups
Group A: ExercisingActive comparator 1 intervention
Group B: Non-exercisingNo intervention 0 interventions

Trial outcomes

Primary outcomes

1

DNA methylation status of adipocytokines in obese individuals

DNA methylation of 20 preselected adipocytokines using "Amplicon sequencing-based methylation profiling" will be measured in fat tissue collected from obese individuals who are scheduled for bariatric surgery before and after 12 weeks of aerobic exercise training.

Time frame
Month 24-30

Secondary outcomes

1

Vascular health biomarkers in obese individuals before and after exercise training

Serum or plasma levels (pg/ml) of biomarkers of vascular function such as endothelin-1, cyclic guanosine monophosphate (cGMP), and endothelial cell adhesion molecules (soluble intracellular adhesion molecules, soluble vascular cell adhesion molecules, soluble platelet and endothelial cell adhesion molecules, soluble E-selectin, and soluble P-selectin).

Time frame
Month 24-30
2

Flow induced dilation

Measuring reactivity of brachial artery and isolated arterioles from adipose tissues

Time frame
Month 1-20

Other outcomes

Sponsors and contacts

Click on the lead sponsor to view all of their trials.

University of Illinois at Chicago

Lead sponsor

National Heart, Lung, and Blood Institute (NHLBI)

Collaborator

Source ClinicalTrials.gov
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