DNA Repair Activity in the Skin of Day and Night Shift Workers
ConditionSkin Cancer
Trial statusRecruiting
Trial phaseNot applicable
Trial typeInterventional
Biological sexAll
Age18-40
SponsorWright State University
About this trial
The purpose of this study is to test how the skin of night shift workers responds to artificial sunlight (ultraviolet B radiation; UVB) at two different times of the day in comparison to normal day shift workers. After the skin biopsies are obtained, they will be brought to the laboratory to be exposed to UVB radiation and to measure UVB responses.
Eligibility criteria
This trial accepts healthy volunteersQualifiers
Male/Female
Fair skin (Fitzpatrick types I and II)
Age 18 to 40
Able to comprehend procedures/risks
Disqualifiers
Known photosensitivity
Currently on photosensitizing medications
Diabetes Mellitus
On any hormonal agents (e.g., birth control pills)
Trial design
Design model
Parallel
Treatments tested in this trial
Ultraviolet B Radiation
RadiationOne skin punch biopsy will be taken to the laboratory for exposure to ultraviolet B radiation. After 1-hour incubation, the biopsies will be bisected in half.
No Ultraviolet B Radiation
Other interventionOne skin punch biopsy will be taken to the laboratory and will be kept as a non-irradiated control.
Treatment groups
48 Participants
are divided into 2 treatment groupsGroup A: Day Shift Work ScheduleExperimental treatment 2 interventions
Group B: Night Shift Work ScheduleExperimental treatment 2 interventions
Trial outcomes
Primary outcomes
1
Expression level of the DNA repair factor XPA at two times of the day in the skin of day and night shift workers.
Reverse transcription quantitative PCR (RT-qPCR) and immunohistochemistry will be used to measure XPA expression at the mRNA and protein level, respectively, in skin samples obtained at 8 am and 4 pm.
Time frame
After 1 hour incubation from ultraviolet B radiation exposure.2
Expression levels of core clock genes at two times of the day in the skin of day and night shift works.
Reverse Transcription Quantitative PCR (RT-qPCR) is used to measure the core circadian clock gene expression at the mRNA level in the skin samples obtained at 8 AM and 4 PM.
Time frame
After 1 hour incubation from ultraviolet B radiation exposure.3
Activity of the nucleotide excision repair (NER) system at two times of the day in day and night shift workers.
A biochemical assay of nucleotide excision repair is used to measure NER in the skin samples obtained at 8 AM and 4 PM.
Time frame
After 1 hour incubation from ultraviolet B radiation exposure.4
Activation of DNA damage kinase signaling pathways at two times of the day in the skin of day and night shift workers.
Immunohistochemistry is used to measure DNA damage kinase signaling (phosphorylated Chk1 and p53) in the skin samples obtained at 8 AM and 4 PM.
Time frame
After 1 hour incubation from ultraviolet B radiation exposure.Sponsors and contacts
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Source ClinicalTrials.gov