Effect of Pranayamic Breathing and Tongue Exercises on Oral Microbiota in Children

ConditionsMouth Breathing Oral Microbiota

Trial statusRecruiting

Trial phaseNot applicable

Trial typeInterventional

Biological sexAll

Age7-12

SponsorGulcin Ozsen

About this trial

This study aims to evaluate the effects of pranayamic breathing techniques and tongue exercises on oral microbiota in children aged 7-12 years. Children will be grouped according to their breathing patterns (mouth or nasal breathing), and a structured breathing and tongue exercise program will be applied. Saliva samples will be collected before and after the intervention to analyze changes in oral microbiota. The study will include approximately 80 participants and is expected to last for 6 months. There are no anticipated risks for participants. The findings of this study may contribute to a better understanding of the relationship between breathing patterns, oral exercises, and oral health in children.

Eligibility criteria

This trial accepts healthy volunteers

Qualifiers

Children aged 7-12 years with either mouth breathing or nasal breathing patterns

Willingness of both the child and their legal guardian to participate in the study

Children who are cooperative and able to comply with the exercise protocol

Disqualifiers

Refusal of the child or legal guardian to participate in the study

Presence of active dental caries or periodontal disease

Presence of any systemic disease or regular medication use

Use of orthodontic appliances

Trial design

Design model

Parallel

Treatments tested in this trial

Pranayamic Breathing and Tongue Exercises
Behavioral
Participants will perform a structured program including pranayamic breathing techniques and tongue exercises, and, in selected subgroups, tongue cleaning. The program will be applied regularly over the study period, and saliva samples will be collected before and after the intervention to assess changes in oral microbiota.

Treatment groups

80 Participants

are divided into 2 treatment groups

Group A: Mouth Breathing GroupExperimental treatment 1 intervention

Group B: Nasal Breathing GroupExperimental treatment 1 intervention

Trial outcomes

Primary outcomes

Change in oral microbiota alpha diversity as measured by the Shannon diversity index from 16S rRNA gene sequencing of saliva samples

This outcome evaluates the effect of the intervention (pranayamic breathing exercises, tongue exercises, and tongue-cleaning practice) on the diversity of the oral microbiota. Alpha diversity will be assessed from saliva samples using 16S rRNA gene sequencing and quantified using the Shannon diversity index. The mean Shannon index at baseline will be compared with the value after the 8-week intervention, and the change from baseline will be reported.

Time frame

8 weeks

Secondary outcomes

Salivary Nitric Oxide Levels

Salivary nitric oxide levels will be measured using test strips before and after the intervention.

Time frame

8 weeks

Sponsors and contacts

Click on the lead sponsor to view all of their trials.

Gulcin Ozsen

Lead sponsor

Istanbul Health and Technology University

Sponsor institution

Source ClinicalTrials.gov

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