Effect of Resveratrol Supplementation on Exercise Performance and Recovery

Venous Blood Sample Collection - Creatine Kinase

Blood samples will be obtained at V2, PRE-, IP, 24H, 48H, and 72H using a 21-gauge vacutainer or 23-gauge butterfly needle from a superficial forearm vein. Up to 2 unsuccessful attempts in one arm, and with permission, a third attempt in the opposite arm, will be made. Three failed attempts will terminate the draw. Issues with blood collection devices will not remove participants from the study. All draws will follow aseptic conditions, with skin disinfected before insertion and the puncture site bandaged afterward. A total of 8mL of blood (slightly less than 2 tsp) will be collected at each time point (V2 \& V6-V13) in one 4mL BD Vacutainer™ SST. Serum tubes will be inverted, clotted at room temperature for 30 minutes, centrifuged for 10 minutes at 4000rpm, aliquoted, and frozen at -80°C within one hour. Serum will be used to determine creatine kinase (CK) concentrations, presented as U/L.

Venous Blood Sample Collection - C-Reactive protein

Blood samples at V2, PRE-, IP, 24H, 48H, and 72H will be obtained using a 21-gauge vacutainer or 23-gauge butterfly needle from a superficial forearm vein. A maximum of 2 unsuccessful attempts per arm will be allowed, with a third attempt permitted in the opposite arm if agreed upon. Three failed attempts will terminate the draw. Issues with blood collection devices will not disqualify participants from the study. All blood draws will follow aseptic conditions, with the skin disinfected before insertion and the puncture site covered with a bandage. A total of 8mL of blood (slightly less than 2 tsp) will be collected at each time point (V2 \& V6-V13) in one 4mL BD Vacutainer™ SST. Serum tubes will be inverted, clotted at room temperature for 30 minutes, centrifuged for 10 minutes at 4000rpm, aliquoted, and frozen at -80°C within one hour of collection. Serum will be used to determine C-reactive protein (CRP) concentrations. and presented at U/L.

Venous Blood Sample Collection - CBC

Blood samples at V2, PRE-, IP, 24H, 48H, and 72H will be obtained using a 21-gauge vacutainer needle or 23-gauge butterfly needle from a superficial forearm vein. A maximum of two unsuccessful attempts per arm will be allowed, with a third attempt in the opposite arm if agreed upon. Three failed attempts will terminate the blood draw. Issues with blood collection devices will not disqualify participants from continuing the study. All blood draws will follow aseptic procedures, with the skin disinfected before insertion and the puncture site covered with a bandage. A total of 8mL of blood (slightly less than 2 tsp) will be collected at each time point (V2 \& V6-V13) in one 4mL K2EDTA plasma tube (EDTA). EDTA samples will be analyzed same day using a Sysmex XN-450 Automated Hematology Analyzer, and used to measure hematocrit, hemoglobin, MCV, MCH, MCHC, total and differential blood cell counts, and platelets in-house at the Cellular Exercise Physiology Laboratory.

Flow Mediated Dilation (FMD)

Subjects will rest in a quiet, dimly lit laboratory in a semi-recumbent position for 15 minutes before testing. Automated sphygmomanometry will measure resting systolic blood pressure (SBP), diastolic blood pressure (DBP), and heart rate (HR) on the left arm, with mean arterial pressure (MAP) calculated. Brachial artery diameter and blood velocity will be assessed using duplex Doppler ultrasound (GE Logiq P9) with a 5-MHz transducer. A tourniquet cuff will be placed around the right forearm to induce reactive hyperemia. Baseline measurements will be recorded for 2 minutes, followed by 5 minutes of arterial occlusion at 240 mmHg, and 3 minutes post-deflation. Ultrasound settings will remain consistent, with scans performed by the same technician and saved via video capture software (Snagit). Offline analysis will use FMD Studio, with parameters calculated via custom macros in Microsoft Excel. FMD will be assessed at V2, and PRE.

Hydration Status

Upon arriving to the lab, participants will be given a sample cup to take to the restroom and provide a urine sample, so that hydration may be assessed. After providing a urine sample, a member of the research team will measure urine specific gravity (USG) using a refractometer to ensure participants are euhydrated (1.002-1.025). If dehydrated (\>1.025), they will be asked to consume 16 oz of water and be retested after waiting 15 minutes post ingestion. If hyperhydrated (\<1.002) their visits will be rescheduled. Hydration status will be checked immediately upon arrival to the laboratory for V2 and each experimental trial (V6-V13). Urine samples will be disposed of according to UCF policy.

Sleep Quality Questionnaire

Participants will be asked to fill out a brief 7 question sleep questionnaire consisting of questions pertaining to their sleep and sleep quality within the last 24 hours. Participants will complete the SQQ immediately upon arriving for each experimental trial (V6-V13)

Diet and Dietary Log

Participants will be asked to maintain two separate 5-day dietary logs using the "MyFitnessPal" application on their own cellular or computer device. Participants will be asked to begin each 5-day log 24 hours before ET1 and ET2 and to maintain the log by tracking all food and beverage consumption to the best of their ability for the 5 days until the completion of the 72H follow up visits (visits 9 \& 13, respectively). Additionally, participants will be asked to keep their food and beverage consumption during the two 5-day periods as consistent as possible and to match macronutrient (fats, carbohydrates, and proteins) consumption between the two periods to the best of their ability. Participants refusing to voluntarily report food and beverage consumption will be withdrawn from the study.

Anthropometric Assessments:

During V2, participants will complete anthropometric assessments of height, weight, and body composition. Height and weight will be assessed using a stadiometer and scale (Health-o-meter Professional Patient Weighing Scale, Model 500 KL, Pelstar, Alsip, IL, USA). Body composition will be assessed using bioelectrical impedance analysis (BIA; InBody 770, Biospace Co, Ltd. Seoul, Korea) or bioelectrical impedance spectroscopy analyzer (Sozo; Carlsbad, CA, USA). Participants will be asked to void their bladder, and remove shoes, socks, and all jewelry for this assessment. They will also be asked to be eight hours fasted and well-hydrated as determined by USG analysis. There are no risks or discomforts associated with the BIA analysis.