Effects of Fibres Combined With Probiotics on Uric Acid in an ex Vivo Fermentation Model
ConditionHyperuricemia
Trial statusRecruiting
Trial phaseNot listed
Trial typeObservational
Biological sexAll
Age18-80
SponsorÖrebro University, Sweden
About this trial
In this study, we will collect faecal samples from individuals with hyperuricaemia (assessed by blood test) and perform in vitro faecal fermentation studies to assess how probiotics in combination with fibres affect urate metabolism in these faecal samples.
Eligibility criteria
This trial does not accept healthy volunteersQualifiers
Signed informed consent prior to any study-related procedures
Age 18-80 years old
BMI range 18.5-35 kg/m2
Blood uric acid above 0.36 mmol/L (6 mg/dl)
Disqualifiers
Diagnosis of type 1 and/or type 2 diabetes
Diagnosed inflammatory bowel disease (IBD)
Current diagnosis of psychiatric disease/s or syndromes
Current diagnosis of neurodegenerative disease
Trial population
Hyperuricaemic adults
Trial design
Design model
Other
Time perspective
Other
Treatments tested in this trial
Not listed
Trial groups
No trial groups listed
Trial outcomes
Primary outcomes
1
Effect of three different beta-glucans in combination with two probiotic strains on urate levels in faecal fermentation supernatants using stool samples from hyperuricaemic individuals
Freshly collected faecal samples will be diluted and incubated with different beta-glucans and two probiotic strains for 24 hours, supernatants will be analysed for urate levels
Time frame
24 hoursSecondary outcomes
1
Effect of three different beta-glucans in combination with two probiotic strains on upstream and downstream metabolites of urate in faecal fermentation supernatants using stool samples from hyperuricaemic individuals
Freshly collected faecal samples will be diluted and incubated with different beta-glucans and two probiotic strains for 24 hours, supernatants will be analysed for urate levels
Time frame
24 hours2
Effect of three different beta-glucans in combination with two probiotic strains on on short-chain fatty acids (SCFA) in faecal fermentation supernatants using stool samples from hyperuricaemic individuals
Freshly collected faecal samples will be diluted and incubated with different beta-glucans and two probiotic strains for 24h, supernatants will be analysed for short-chain fatty acids (SCFA)
Time frame
24 hoursOther outcomes
1
Effect of three different beta-glucans in combination with two probiotic strains on general microbial activity (e.g., pH) of the faecal fermentation extracts using stool samples from hyperuricaemic individuals
Freshly collected faecal samples will be diluted and incubated with different beta-glucans and two probiotic strains for 24h, supernatants will be analysed for general microbial activity (e.g., pH)
Time frame
24 hours2
Effect of three different beta-glucans in combination with two probiotic strains on the faecal microbiome of the faecal fermentation extracts using stool samples from hyperuricaemic individuals
Freshly collected faecal samples will be diluted and incubated with different beta-glucans and a probiotic product, and the faecal microbiome will be analysed in the pellets (by sequencing)
Time frame
24 hours3
Effect of three different beta-glucans in combination with two probiotic strains on the metagenetic activity (using transcriptomics) of the faecal fermentation extracts using stool samples from hyperuricaemic individuals
Freshly collected faecal samples will be diluted and incubated with different beta-glucans and a probiotic product for 24h, pellets will be analysed regarding metagenetic activity (from transcriptomics)
Time frame
24 hours4
Effect of three different beta-glucans in combination with two probiotic strains on the metagenetic activity (using metabolomics) of the faecal fermentation extracts using stool samples from hyperuricaemic individuals
Freshly collected faecal samples will be diluted and incubated with different beta-glucans and two probiotic strains for 24h, pellets will be analysed regarding metagenetic activity (from metabolomics) using stool samples from hyperuricaemic individuals
Time frame
24 hoursSponsors and contacts
Click on the lead sponsor to view all of their trials.
Örebro University, Sweden
Lead sponsor
University of Copenhagen
Collaborator
Source ClinicalTrials.gov