Effects of Protein Type and Mineral Form on Amino Acid Availability

ConditionProtein Metabolism

Trial statusNot yet recruiting

Trial phaseNot applicable

Trial typeInterventional

Biological sexAll

Age18-35

SponsorMaastricht University Medical Center

About this trial

To compare postprandial plasma amino acid availability, expressed as incremental area under the curve (iAUC), over a 6 hour period following ingestion of 25 g of different protein isolates in healthy, young adults. The primary objective is divided into the following 2 sub-studies, each with 3 comparisons:

* Sub-study 1: whey protein, calcium caseinate, sodium caseinate. * Sub-study 2: calcium caseinate, magnesium caseinate, potassium caseinate.

Eligibility criteria

This trial accepts healthy volunteers

Qualifiers

Male or female sex

Aged between 18 - 35 years inclusive

BMI between 18 - 30 kg/m2

Healthy, recreationally active (exercise at least once per two weeks and a maximum of four days per week)

Disqualifiers

None

Trial design

Design model

Crossover

Treatments tested in this trial

Protein beverage
Dietary supplement
25 g protein isolate, dissolved in 500 mL water

Treatment groups

16 Participants

are divided into 5 treatment groups

Group A: Whey proteinActive comparator 1 intervention

Group B: Sodium caseinateExperimental treatment 1 intervention

Group C: Calcium caseinateExperimental treatment 1 intervention

Group D: Magnesium caseinateExperimental treatment 1 intervention

Group E: Potassium caseinateExperimental treatment 1 intervention

Trial outcomes

Primary outcomes

Six-hour postprandial plasma total amino acid availability following ingestion of 25 grams of different protein isolates in healthy, young adults

Plasma total amino acid availability is expressed as the incremental area under the curve (iAUC) and compared between whey protein, calcium caseinate and sodium caseinate (sub-study 1) and between calcium caseinate, magnesium caseinate and potassium caseinate (sub-study 2).

Time frame

Six hours

Secondary outcomes

Plasma essential amino acid concentrations

The summed measurement of histidine, leucine, lysine, methionine, phenylalanine, threonine, tryptophan and valine concentrations

Time frame

Essential amino acid concentrations are derived from blood samples taken before beverage ingestion and during the six-hour postprandial time-period (at 15, 30, 45, 60, 75, 90, 120, 150, 180, 210, 240, 300 and 360 minutes following beverage ingestion).

Non-essential amino acid concentrations

The summed measurement of alanine, arginine, aspartic acid, cysteine, glutamic acid, glycine, serine, tyrosine and valine concentrations

Time frame

Non-essential amino acid concentrations are derived from blood samples taken before drink ingestion and during the six-hour postprandial time-period (at 15, 30, 45, 60, 75, 90, 120, 150, 180, 210, 240, 300 and 360 minutes following beverage ingestion).

Plasma amino acid kinetics

Compare peak plasma amino acid concentrations and the time to reach these peak concentrations of all individually measured amino acids

Time frame

During the six-hour postprandial period

Circulating mineral concentrations

Calcium, magnesium, sodium and potassium concentrations

Time frame

During the six-hour postprandial period

Other outcomes

Circulating parathyroid concentrations

Parathyroid hormone concentrations

Time frame

During the six-hour postprandial period

Sponsors and contacts

Click on the lead sponsor to view all of their trials.

Maastricht University Medical Center

Lead sponsor

FrieslandCampina N.V.

Collaborator

Source ClinicalTrials.gov

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