EKLF Gene Expression in β-thalassemia

Conditionβ-thalassemia

Trial statusNot yet recruiting

Trial phaseNot listed

Trial typeObservational

Biological sexAll

Age5-80

SponsorRofaida Hassan Ahmed

About this trial

1. Studying the effect of expression pattern of EKLF gene in β-thalassemic patients. 2. Detecting the correlation between the gene expression of EKLF and the clinical phenotype of β-thalassemic patients.

Eligibility criteria

This trial accepts healthy volunteers

Qualifiers

patients with β-thalassemia (major and intermedia).

patients are of both sexes (male or female) at any age

Disqualifiers

patients with any other types of hemolytic anaemia

Trial population

Patients \> 5 years diagnosed with β-thalassemia including both thalassemia major and intermedia

Trial design

Design model

Case-control

Time perspective

Retrospective

Treatments tested in this trial

PCR
Diagnostic test
Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) for detection of EKLF: * The method of total RNA extraction: TRIZOL and TRIZOL LS. * The purity and concentration of the RNA will be measured using Nano Drop 2000 instument. * cDNA will be done with primers using thr Goscript Reverse Transcription System

Treatment groups

150 Participants

are divided into 3 treatment groups

Group A: Group I1 intervention

Group B: Group 21 intervention

Group C: Group 31 intervention

Trial outcomes

Primary outcomes

study the expression pattern of EKLF gene in β-thalassemic patients, work by measuring mRNA levels

study the expression pattern of EKLF gene by Real-time polymerase chain reaction (RT-qPCR) in β-thalassemic patients

Time frame

Baseline

Secondary outcomes

study the correlation between the gene expression of EKLF and the clinical phenotype of β-thalassemic patients.

Time frame

Baseline

Locations

This trial has no locations.

Sponsors and contacts

Click on the lead sponsor to view all of their trials.

Rofaida Hassan Ahmed

Lead sponsor

Assiut University

Sponsor institution

Source ClinicalTrials.gov

This trial is not recruiting at the moment. You can still explore other options: