Erythrocyte Transport of Lactate During Exercise (TELE Project)

ConditionEnergy Metabolism

Trial statusRecruiting

Trial phaseNot applicable

Trial typeInterventional

Biological sexAll

Age18-30

SponsorTechnical University of Madrid

About this trial

The goals of this clinical trial are:

1. to study lactate kinetic between plasma and erythrocytes during an intervallic exercise and its subsequent recovery, considering the blood pH, the genotype for the T1470A polymorphism of the SLC16A1 gene (rs1049434), and the amount of MCT1 in erythrocytes membrane; 2. to analyze the levels of MCT1 in the erythrocytes membrane according to training status and genotype for the T1470A polymorphism of SLC16A1 (rs1049434).

For this, the project will have two phases:

* In phase I, trained participants will perform one maximal incremental test and one intervallic submaximal test with a final active recovery. * Phase II, levels of the MCT1 protein in the erythrocyte membrane will be quantified from trained and sedentary participants.

Eligibility criteria

This trial accepts healthy volunteers

Qualifiers

BMI between 18 and 25 kg/m2

Not smoker

In the case of trained participants: training at least 5 hours/week, of which at least 3 must be aerobic/anaerobic endurance training. Presentation of a medical certificate attesting to their medical fitness (capability) to perform intense physical exercise.

In the case of sedentary participants, less than 1 day of training per week or less than 2 hours of structured exercise per week.

Disqualifiers

Presenting any pathology that could impede the correct performance of the tests, or could influence the main study variables.

In the case of the female sample, having irregular menstrual cycles, oligomenorrhea or amenorrhea.

Trial design

Design model

Single group

Treatments tested in this trial

Intervallic submaximal exercise test
Other intervention
Intervallic submáximal exercise test on a cycle ergometer: 5 min warm-up at 80% of First Lactate Threshold (LT1), and subsequent sets of 1.5 min at 20% above the Second Lactate Threshold (LT2) alternated with recoveries of 5 min at 80% of LT1, ending with a 20 min recovery at 80% of LT1.

Treatment groups

84 Participants

are divided into 1 treatment group

Group A: Intervallic submaximal testExperimental treatment 1 intervention

Trial outcomes

Primary outcomes

Plasma-Erythrocyte lactate difference

Difference between lactate concentration inside the erythrocytes and lactate concentration in plasma, expressed in mMol/L (Millimoles Per Liter), from venous blood extracted during the intervallic test from the antecubital vein.

Time frame

Through Phase I of the project, only in the intervallic submaximal test. Average of 3 years

Level of Monocarboxylate Transporter 1 in erythrocytes membrane

Protein abundance of the monocarboxylate transporter 1 in erythrocyte membranes. It is measured using flow cytometry, from venous blood extracted from the forearm in rest state.

Time frame

Through study completion, average of 5 years, an average of 2 months per participant

Secondary outcomes

Erythrocyte lactate concentration

Lactate concentration inside the erythrocytes, expressed in mMol/L (Millimoles Per Liter), measured in erythrocytes extracted from processed venous blood. Lactate concentration is measured in the supernatant obtained after the lysis and centrifugation of the solid part of the blood (mainly erythrocytes), separated by centrifugation. Venous blood samples are extracted during the intervallic test from the antecubital vein.

Time frame

Through Phase I of the project, only in the intervallic submaximal test. Average of 3 years

Plasma lactate concentration

Lactate concentration, expressed in mMol/L (Millimoles Per Liter), measured in the plasma of centrifugated venous blood, using a lactate analyzer. Lactate concentration is measured in the plasma obtained from venous blood extracted during the intervallic test from the antecubital vein.

Time frame

Through Phase I of the project, only in the intervallic submaximal test. Average of 3 years

Blood lactate concentration

Lactate concentration, expressed in mMol/L (Millimoles Per Liter), measured from whole venous blood extracted during the intervallic test from the antecubital vein.

Time frame

Through Phase I of the project, only in the intervallic submaximal test. Average of 3 years

Blood pH

Level of blood pH measured in venous blood extracted during the intervallic test from the antecubital vein.

Time frame

Through Phase I of the project, only in the intervallic submaximal test. Average of 3 years

Other outcomes

Genotype for the rs1049434 polymorphism of the SLC16A1 (MCT1) gene

Genotype for the Asp490Glu (T1470A) (rs1049434) polymorphism of the SLC16A1 (MCT1) gene, resulting in one of the following three profiles: TT (Arg/Arg), TA (Arg/Glu) or AA (Glu/Glu)

Time frame

Through sample recruitment, average of 5 years, an average of 1 month per participant

Physical Activity level

The IPAQ self-reported questionnaire will be used to quantify physical activity levels.

Time frame

Through sample recruitment, average of 5 years, an average of 1 month per participant

Sponsors and contacts

Click on the lead sponsor to view all of their trials.

Technical University of Madrid

Lead sponsor

Source ClinicalTrials.gov

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