The host immune response to microorganisms is primarily recognized by pattern recognition receptors (PRRs), including toll-like receptors and nucleotide-binding oligomeric domain (NOD) receptors (NLRs). The aim of this study was to examine the relationship between periodontal disease and NOD in serum and saliva samples.
systemically healthy
clinical diagnosis of periodontal health
clinical diagnosis of periodontitis
clinical diagnosis of gingivitis
• history of regular use of systemic antibiotics anti-inflammatory, or antioxidant drugs (previous 3 months)
nonsurgical periodontal treatment (previous 6 months)
surgical periodontal treatment (previous 12 months)
presence of<10 teeth
Single group
Saliva and Serum sampling
Other interventionSaliva were collected to analyze the selected markers as unstimulated samples during the early hours of the day. The saliva was centrifuged and then transferred into Eppendorf tubes. Venous puncture was performed after saliva collection and 5 mL of blood samples were collected by qualified staff from each participant.Saliva and serum were then stored at -80 °C until analysis.
Saliva and Serum sampling
Other interventionELISA method will be used to measure IL-10 and IL-6 values in collected saliva and serum samples. For ELISA analysis, a commercial kit (Sunred Bio, Shanghai, China) pre-coated with human selected antibodies will be used.
Pocket probing depth
Measurement of the depth of a sulcus or periodontal pocket determined by measuring distance from a gingival margin to the base of the sulcus or pocket with a calibrated periodontal probe.
Clinical attachment level
Clinical attachment level (or loss, CAL) is a more accurate indicator of the periodontal support around a tooth than probing depth alone. CAL is measured from a fixed point on the tooth that does not change, the CEJ. To calculate CAL, two measurements are needed: distance from the gingival margin to the CEJ and probing depth. In recession: probing depth (+) gingival margin to the CEJ (add). In tissue overgrowth: probing depth (-) gingival margin to the CEJ (subtract)
Bleeding on probing
Referring to bleeding that is induced by gentle manipulation of the tissue at the depth of the gingival sulcus, or interface between the gingiva and a tooth.
Saliva and serum samples processing and analyses
Saliva and serum samples obtained for each patient were used for cytokine analysis. Prepared samples were analyzed for NOD1, NOD2, IL-10 and IL-6 using commercial ELISA kits
Click on the lead sponsor to view all of their trials.
This trial is not recruiting at the moment. You can still explore other options:
