Clear cell renal cell carcinoma is diagnosed by imaging, sometimes associated with biopsy. This diagnosis is expensive, invasive and sometimes late. The development of a simple biological test for diagnosis is essential. Exosomes are 30 to 150 nm membrane vesicles secreted into the extracellular space by various living cells. These exosomes can be isolated from biological fluids, including urine. The recent study of urinary exosomes is a promising topic for analyzing tumor markers in urine. The investigator's goal is to develop a reliable technique for detecting tumor exosomes in urine in patients with clear cell renal cell carcinoma. The analysis of urinary exosomes could provide a new liquid biopsy tool for the early diagnosis of clear cell renal cell carcinoma.
All patients with renal mass requiring surgery (partial or total nephrectomy)
Social security affiliation
Signed informed consent
Unscheduled nephrectomy
Insufficient volume of urine sample (< 100 ml)
Patients with a urinary catheter
Patients under court-ordered guardianship or curators
60 patients and 40 control will be included.
Cohort
Prospective
Urinary sample
Biological/VaccineUrinary sample (100 ml) will be collected in the urology department to analysis urinary exosomes.
Number of patients (with clear cell kidney cancer) with CD9+ and CA9+ exosomes by electron microscopy technique
Measured by analysis in electron microscopy technique of urinary sample.
Percentage of CD9+ /CA9+ exosomes by electron microscopy technique (%) for patients with clear cell kidney cancer
Measured by analysis in electron microscopy technique of urinary sample.
Percentage of CD9+ /CA9+ exosomes by electron microscopy technique (%) for control patients
Measured by analysis in electron microscopy technique of urinary sample.
Percentage of CD9+ /CA9+ exosomes by flow cytometry (%) for patients with clear cell kidney cancer
Measured by analysis in flow cytometry of urinary sample.
Percentage of CD9+ /CA9+ exosomes by flow cytometry (%) for control patients
Measured by analysis in flow cytometry of urinary sample.
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