Exhaled Breath Condensate Analysis in Mechanically Ventilated Patients
Trial statusRecruiting
Trial phaseNot listed
Trial typeObservational
Biological sexAll
Age18+
SponsorThe Institute of Molecular and Translational Medicine, Czech Republic
About this trial
Mechanically ventilated intensive care patients will be sampled for a small amount of exhaled breath condensate from the ventilator circuit and for venous blood.
Proteomic analysis of the exhaled breath condensate will be performed using mass spectrometry and in the blood sample, corresponding changes in the DNA, RNA, proteins, and metabolites will be studied. Resulting profiles will be correlated with routinely monitored parameters in order to identify patterns corresponding to various pathologies in order to enable their early detection.
Eligibility criteria
This trial does not accept healthy volunteersQualifiers
None
Disqualifiers
None
Trial population
Mechanically ventilated intensive care patients with or without acute hypoxemic respiratory failure
Trial design
Design model
Cohort
Time perspective
Prospective
Treatments tested in this trial
Proteomic analysis of exhaled breath condensate
Diagnostic testMechanically ventilated intensive care patients will be sampled for exhaled breath condensate from the ventilator circuit.
Treatment groups
200 Participants
are divided into 2 treatment groupsGroup A: Mechanically ventilated patients with primary or secondary lung injury1 intervention
Group B: Mechanically ventilated patients without lung injury1 intervention
Trial outcomes
Primary outcomes
1
Comparison of proteomic patterns of the exhaled breath condensate in mechanically ventilated intensive care patients.
Proteomic patterns of the exhaled breath condensate will be studied in mechanically ventilated intensive care patients. The results will be analysed and compared with the aim to identify patterns specific for the pathological conditions.
Time frame
January 2024 - December 2029Secondary outcomes
1
Correlation of exhaled breath condensate patterns with molecular biological findings in venous blood - DNA
Venous blood sample will be analysed employing targeted analyses of DNA. The results will be correlated to proteomic patterns obtained from the exhaled breath condensate.
Time frame
January 2024 - December 20292
Correlation of exhaled breath condensate patterns with molecular biological findings in venous blood - RNA
Venous blood sample will be analysed employing targeted analyses of RNA. The results will be correlated to proteomic patterns obtained from the exhaled breath condensate.
Time frame
January 2024 - December 20293
Correlation of exhaled breath condensate patterns with molecular biological findings in venous blood - proteins
Venous blood sample will be analysed employing targeted analyses of proteins. The results will be correlated to proteomic patterns obtained from the exhaled breath condensate.
Time frame
January 2024 - December 20294
Correlation of exhaled breath condensate patterns with molecular biological findings in venous blood - metabolites
Venous blood sample will be analysed employing targeted analyses of metabolites. The results will be correlated to proteomic patterns obtained from the exhaled breath condensate.
Time frame
January 2024 - December 2029Sponsors and contacts
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Source ClinicalTrials.gov