Fetal Cell Receptors Repertoire

ConditionsVenous Ulcer Sickle Cell Ulcer Diabetic Ulcer Post-partum Women Mixed Ulcer

Trial statusRecruiting

Trial phaseNot applicable

Trial typeInterventional

Biological sexAll

AgeNot listed

SponsorAssistance Publique - Hôpitaux de Paris

About this trial

The purpose of this study is to describe the transcriptomic profile of foetal cells in post-partum and more specifically to determine which chemokine receptors are overexpressed in foetal cells in post-partum women with wounds To do so, the investigators will isolate foetal cells from the peripheral blood of healthy controls post partum women as well as from post partum women with skin ulcers and then perform RNA sequencing.

Eligibility criteria

This trial accepts healthy volunteers

Qualifiers

Adult women,

Post-partum: having been pregnant for any length of time,

Having signed a free and informed consent form,

Primiparous or multiparous,

Disqualifiers

Minors (for patients)

Under court protection, curatorship, guardianship (for patients)

Immunocompromised patients for any reason whatsoever

Refusal of consent

Trial design

Design model

Parallel

Treatments tested in this trial

Saliva sampling
Other intervention
HLA genotyping. The technique should allow to identify, for children's, a paternal HLA antigen not shared with the mothers.
Blood sampling
Other intervention
Maternal Blood samples will be incubated with the appropriate antibody, targeting the microchimeric fetal cells of each patient, as well as with a cell viability marker (DAPI). The samples were then be processed through the BD FACS Aria III to sort the fetal cells, The following steps - RNA extraction, quality control, retrotranscription, preparation of the library, sequencing and transcriptomic analysis - will be carried out according to the Smart-seq3 protocol. The data will be sent for in-depth analysis and confirmation of the results. Additional functional experiments may also be carried out.
Interviews
Other intervention
V2 and/or V3
Clinical examination
Other intervention
V2 and/or V3

Treatment groups

160 Participants

are divided into 3 treatment groups

Group A: PatientsOther 4 interventions

Group B: Patient "Controls group "Other 4 interventions

Group C: ChildrenOther 1 intervention

Trial outcomes

Primary outcomes

Transcriptomic analysis by single cell sequencing

Transcriptomic analysis by single cell RNA sequencing (Smart-seq3 protocol) of fetal cells sorted from peripheral blood

Time frame

Month 1 up to month 5

Sponsors and contacts

Click on the lead sponsor to view all of their trials.

Assistance Publique - Hôpitaux de Paris

Lead sponsor

URC-CIC Paris Descartes Necker Cochin

Collaborator

Source ClinicalTrials.gov

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