Flow Cytometric Immune Monitoring of B. Afzelii-Specific T Lymphocytes
Trial statusNot yet recruiting
Trial phaseNot listed
Trial typeObservational
Biological sexAll
Age18+
SponsorUniversity Hospital, Strasbourg, France
About this trial
Lyme borreliosis diagnosis currently relies on clinical assessment and detection of Borrelia-specific antibodies. However, serology does not allow reliable assessment of active infection. Evaluation of the cellular immune response through Borrelia-specific T lymphocyte monitoring may provide complementary diagnostic and prognostic information.
This study aims to assess Borrelia afzelii-specific T-cell responses using multiparameter flow cytometry after stimulation with standardized peptide pools. This approach enables the characterization of CD4+ and CD8+ T cells, including polyfunctional immune responses.
The diagnostic and prognostic value of this immunomonitoring strategy will be evaluated in patients with acrodermatitis chronica atrophicans, a late manifestation of Lyme borreliosis associated with B. afzelii.
Eligibility criteria
Qualifiers
Age ≥ 18 years
Subject informed of the results of the prior medical examination
Subject informed about the objectives, procedures, and potential risks of the study
Subject affiliated with a health insurance/social security scheme (beneficiary or dependent)
Disqualifiers
Body weight < 50 kg
Pregnancy or breastfeeding
Diabetes mellitus
Immunodeficiency or ongoing immunosuppressive treatment (including oral corticosteroids)
Trial design
Treatments tested in this trial
- Not listed
Trial groups
20 Participants
are grouped into 2 trial groupsSponsors and collaborators
Source ClinicalTrials.gov