Follicular Fluid Exosome miRNA During Oocyte Maturation
ConditionOocyte Maturation
Trial statusRecruiting
Trial phaseNot listed
Trial typeObservational
Biological sexFemale
Age20-45
SponsorChinese University of Hong Kong
About this trial
Folliculogenesis involves the recruitment of primordial follicles into a group of growing follicles in which eventually one follicle is selected, matured and ovulated. Complex endocrine and intra-ovarian paracrine interactions occur to create a changing intra-follicular hormonal milieu suitable for oocyte development. However, this oocyte developmental competence and to undergo fertilization and embryogenesis is impaired in aged women. Diminished ovarian function is generally attributed to decreased quantity and quality of oocytes and their surrounding granulosa cells during ovarian aging, although the underlying mechanisms remain unclear.
The aim of the study is to investigate the miRNA in (follicular fluid) FF exosome in young and aged women and their relationship to egg maturation.
Eligibility criteria
This trial accepts healthy volunteersQualifiers
Women aged 20 to 45;
BMI > 19 & < 35;
Women who is planning to perform invitro fertilization (IVF) together with intracytoplasmic sperm injection (ICSI) for their fertility problems
Disqualifiers
Positive pregnancy test before taking study medications;
Women with BMI>35 kg/m2;
Women with known chromosomal translocation, recurrent abortions or those carrying Fragile X;
Women with endometriosis
Trial population
women undergoing oocyte retrieval treatment
Trial design
Design model
Cohort
Time perspective
Prospective
Treatments tested in this trial
Not listed
Trial groups
No trial groups listed
Trial outcomes
Primary outcomes
1
RNA-sequencing
1\) To identify for the first time FF exosome miRNAs in mature and immature follicles in young and aged women by RNA-sequencing (RNA-seq).
Time frame
through study completion, an average of 2 years2
quantitative real-time polymerase chain reaction
2\) To verify the identified FF exosome miRNAs in mature and immature follicles in young and aged women and investigate their expression in the corresponding granulosa cells by quantitative real-time polymerase chain reaction (qRT-PCR).
Time frame
through study completion, an average of 2 yearsSecondary outcomes
1
fertilization and pregnancy rate
3\) To observe the effect of the identified specific FF exosome miRNAs on oocyte maturation (by IVM) and assess the association of the miRNAs on fertilization and pregnancy rate.
Time frame
through study completion, an average of 2 yearsSponsors and contacts
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Source ClinicalTrials.gov