The purpose of this study is to investigate the role of exosomal microRNAs (miRNAs) in follicular fluid (FF) as biomarkers of ovarian aging and predictors of in vitro fertilization (IVF) outcomes. The goal is to identify noninvasive molecular markers that correlate with oocyte quality and reproductive potential, particularly in women of advanced maternal age.
Women undergoing IVF treatment due to male factor infertility or nonovarian causes.
Age groups: younger women <31 years and older women >38 years.
Regular menstrual cycles.
Willing and able to provide informed consent.
Minors (under 18 years) and women aged 32-37 years
Diagnosis of polycystic ovarian syndrome (PCOS).
History of recurrent pregnancy loss.
Presence of endometriosis.
Potential participants will be screened and identified during their initial consultation and scheduling for IVF treatment at The IVF Center. Medical records and patient history will be reviewed to screen for eligibility based on inclusion and exclusion criteria. Women who meet all inclusion criteria and none of the exclusion criteria will be invited to participate and consented for the study.
Case-only
Prospective
No Interventions
Other interventionNo interventions for participants
Identify exosomal miRNAs using RNA sequencing
Identify differentially expressed exosomal miRNAs in the follicular fluid of younger (\<31 years) versus older (\>38 years) women undergoing IVF using small RNA sequencing.
Validate and assess miRNA for IVF
Validate the expression of selected miRNAs in an independent sample set and assess their correlation with clinical IVF parameters, including oocyte maturity, fertilization, and embryo development.
Determine molecular pathways regulated by age
Use bioinformatic analysis and in vitro functional assays to determine the molecular pathways regulated by age associated miRNAs, particularly those involved in follicular development, hormone signaling, and cellular aging.
Click on the lead sponsor to view all of their trials.
University of Central Florida
Lead sponsor
The IVF Center at Winter Park
Collaborator
