Garden-fresh Foods and Gut Microbiomes
ConditionGut Microbiome
Trial statusRecruiting
Trial phaseNot applicable
Trial typeInterventional
Biological sexAll
Age18-45
SponsorUniversity of Oregon
About this trial
The purpose of this research is to explore what types of microbes are present on garden-fresh versus store-bought fruits and vegetables, as well as how they might affect the human gut microbiome.
Eligibility criteria
This trial accepts healthy volunteersQualifiers
None
Disqualifiers
Body mass index (BMI) greater than or equal to 35 or less than or equal to 18.
Age under 18 or over 45.
Female who is pregnant or lactating.
Irregular bowel movements and/or stool consistency.
Trial design
Design model
Crossover
Treatments tested in this trial
Garden-fresh Produce
Other interventionParticipants are asked to consume the USDA-recommended amounts of fruits and vegetables harvested from their gardens.
Supermarket Produce
Other interventionParticipants are asked to consume the USDA-recommended amounts of fruits and vegetables purchased from a supermarket.
Treatment groups
20 Participants
are divided into 2 treatment groupsGroup A: Garden FirstExperimental treatment 2 interventions
Group B: Supermarket FirstExperimental treatment 2 interventions
Trial outcomes
Primary outcomes
1
Difference in fecal bacterial abundance, as characterized by qPCR with "universal" bacterial primers
ANOVA statistical test of bacterial gene copies (a proxy for absolute abundance) used to compare whether Garden-Fresh Produce and Supermarket Produce interventions differentially affect fecal bacterial abundance.
Time frame
Baseline, 1 week, 3 weeks2
Difference in fecal microbial diversity, as characterized by shotgun metagenomics.
ANOVA statistical test of Shannon index used to compare whether Garden-Fresh Produce and Supermarket Produce interventions differentially affect fecal microbial diversity.
Time frame
Baseline, 1 week, 3 weeks3
Difference in fecal microbial composition, as characterized by shotgun metagenomics.
PERMANOVA statistical test used to compare whether Garden-Fresh Produce and Supermarket Produce interventions differentially affect fecal microbial composition.
Time frame
Baseline, 1 week, 3 weeks4
Presence of differentially abundant microbial taxa, as characterized by shotgun metagenomics.
Negative binomial GLM statistical test used to identify microbial taxa associated with either Garden-Fresh Produce or Supermarket Produce intervention.
Time frame
Baseline, 1 week, 3 weeksSponsors and contacts
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Source ClinicalTrials.gov