Glycocalyx and Oxidative Stress in Endothelial Function (Part 2)
ConditionCoronary Heart Disease
Trial statusRecruiting
Trial phaseNot listed
Trial typeObservational
Biological sexAll
Age18+
SponsorJosip Juraj Strossmayer University of Osijek
About this trial
The primary research objective of the project is to determine the role of eGC in microvascular reactivity in patients with coronary heart disease (CHD) before and after bypass grafting (cross-sectional study 2).The main objective of the project is to increase the excellence and interdisciplinarity of scientific work at MEFOS by connecting researchers from different scientific fields, improving conditions and resources for scientific work, and increasing international cooperation, in line with strategic objective 1: raising scientific excellence. The results of the project could provide pathophysiological insight into the development and maintenance of endothelial dysfunction in CVD, as well as identify new biomarkers for CVD.
Eligibility criteria
This trial does not accept healthy volunteersQualifiers
adult patients of both sexes over 18 years of age
Caucasian
undergoing elective coronary artery bypass grafting (CABG)
provided written informed consent
Disqualifiers
emergency surgery
sepsis or severe systemic inflammation before surgery
severe renal or hepatic insufficiency
immunosuppression
Trial population
Adult patients undergoing elective coronary artery bypass grafting (CABG)
Trial design
Design model
Case-control
Time perspective
Prospective
Treatments tested in this trial
Not listed
Trial groups
35 Participants
are grouped into 1 trial groupGroup A: Coronary Heart Disease (CHD) patients
Trial outcomes
Primary outcomes
1
Endothelial glycocalyx
Measurement of the thickness of endothelial glycocalyx in coronary heart disease patients before and after bypass surgery - measured by Glycocheck
Time frame
Perioperative/PeriproceduralSecondary outcomes
1
Bypass graft sample reactivity
Determine the reactivity of the bypass graft sample in in vitro experiments - measured by isolated vessel setup
Time frame
Baseline, at one time point2
Endothelial glycocalyx soluble markers
Quantification of soluble markers of endothelial glycocalyx from serum or plasma samples in the specified groups - measured by ELISA assays (syndecan-1, syndecan-4, glypican-1, MMP-9)
Time frame
Perioperative/Periprocedural3
Intracellular oxidative stress production
Quantification of intracellular oxidative stress production from peripheral blood mononuclear cells (PBMC) in the specified groups - measured by flow cytometry (DCF-DA and DHE)
Time frame
Perioperative/Periprocedural4
Inflammation
Quantification of inflammation from peripheral blood mononuclear cells (PBMC) in the specified groups - measured by Luminex assay (cytokine profile) and flow cytometry (macrophage frequency)
Time frame
Perioperative/PeriproceduralOther outcomes
1
Blood pressure
Automatic oscillometric measurement of systolic and diastolic blood measurement (mmHg)
Time frame
Perioperative/Periprocedural2
Heart rate
Automatic oscillometric measurement of heart rate (bpm)
Time frame
Perioperative/Periprocedural3
Body Mass Index
Measurement of BMI (kg/m2)
Time frame
Perioperative/Periprocedural4
Body composition
Body composition and body fluid status measurement by a four-terminal portable impedance analyzer. Based on the value of skin resistance (Ohm) empirically derived formulas (the original manufacturer's software) will be used to calculate the estimated Fat Free Mass (FFM kg) and Fat (Fat Mass kg).
Time frame
Perioperative/PeriproceduralSponsors and contacts
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Source ClinicalTrials.gov