How a Single Workout Affects Gut Bugs in Women With Different Fitness Levels and Body Types
Trial statusRecruiting
Trial phaseNot applicable
Trial typeInterventional
Biological sexFemale
Age21-40
SponsorGeorge Washington University
About this trial
This study aims to elucidate the differences in the gut microbiome functional activity and metabolome in adult premenopausal women with distinctive fitness levels and BMIs (with obesity, w/o obesity). The specific aims are as follows:
* Aim 1: To examine the effects of acute aerobic exercise at 60-70% heart rate reserve (HRRmax) for 30 minutes bout on changes in the abundance of SCFA-producing bacteria and their functional downstream metabolic activity. * Aim 2: To examine the effects of acute aerobic exercise at 60-70% HRRmax 30-minute bout on changes in GM-released SCFA concentrations in stool and plasmatic metabolome.
Eligibility criteria
This trial accepts healthy volunteersQualifiers
Biological sex: female
BMI 19 to 24.99 kg/m2 or BMI 30-40 kg/m2
Exercisers: Exercise at least 4 times per week for 60 minutes (a total of 240 minutes per week) on a programmed exercise training either on endurance sports (e.g., running, cycling, triathlon), strength (e.g., powerlifting), or team sports (e.g., rugby, football, soccer)
Non-Exercisers: Sedentary individuals who have not reached the PA guidelines (150 minutes of moderate-intensity physical activity a week, 75 minutes of vigorous-intensity physical activity, or an equivalent combination of moderate and vigorous-intensity physical activity).
Disqualifiers
Biological sex: male
BMI < 19 kg/m2 or > 40 kg/m2
Currently pregnant, < 2 years postpartum, lactating
Currently taking any herbal, fiber, or prebiotic supplement
Trial design
Design model
Single group
Treatments tested in this trial
Aerobic Exercise
Behavioral30-minute exercise bout in a cycle ergometer at 60 to 70% of the Heart Rate Reserve (HRR) and corresponding power (watts).
Treatment groups
40 Participants
are divided into 1 treatment groupGroup A: InterventionExperimental treatment 1 intervention
Trial outcomes
Primary outcomes
1
Gut Microbiome Composition
Shotgun metagenomics will be used to examine the abundance of specific SCFA-producing bacteria in the stool (e.g., Ruminococcus)
Time frame
Before the exercise intervention and immediately after the intervention2
Gut Microbiome Function
Shotgun metagenomics will be used to examine the BGCs (i.e., the genes within the microbial genome that dictate gut microbial functionality) to investigate functional downstream metabolic activity.
Time frame
Before the exercise intervention and immediately after the intervention3
Plasma Metabolome
Gas chromatography and mass spectrometry will be used to measure concentrations of SCFAs in plasma before and after the exercise intervention
Time frame
Before the exercise intervention, immediately after the exercise intervention, and 24 hours before the intervention4
Stool Metabolome
Gas chromatography and mass spectrometry will be used to measure concentrations of SCFAs in stool before and after the exercise intervention
Time frame
Before the exercise intervention, immediately after the exercise intervention, and 24 hours before the interventionSponsors and contacts
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Source ClinicalTrials.gov