Human Skeletal Muscle Response to 5 Days of Bedrest in Young Adults
Trial statusRecruiting
Trial phaseNot applicable
Trial typeInterventional
Biological sexAll
Age18-30
SponsorQueen's University
About this trial
The goal of this intervention trial is to characterize skeletal muscle atrophy in healthy, young adults during short term bedrest. The main questions it aims to answer are:
How much do skeletal muscle volume, strength, and fatigue resistance decline during bedrest? How much does whole-body insulin sensitivity change during bedrest? How do mitochondrial function and protein synthesis change during bedrest?
Participants will undergo the following tests before and after a free-living control period and before and after a 5 day period of strict horizontal bedrest:
* Magnetic resonance imaging of the thigh muscles * Strength testing of the thigh muscles * Insulin sensitivity testing in response to a mixed meal * Exogenous glucose oxidation in response to a mixed meal * Muscle biopsies from the thigh muscles * Blood samples
Eligibility criteria
This trial accepts healthy volunteersQualifiers
Males and females 18-30 years
BMI between 18.5-28 kg/m2
Weight stable (within ±2kg for 6 months)
Generally healthy as assessed by medical and physical activity questionnaires
Disqualifiers
a BMI < 18.5 and > 28 kg/m2
the use of insulin to control blood glucose levels
a history of any cardiovascular, respiratory, metabolic diseases, neuromuscular or bone-wasting diseases
the use of any medication that may affect muscle protein turnover (e.g. androgen or anabolic hormone therapy, chemotherapy)
Trial design
Design model
Single group
Treatments tested in this trial
Bedrest
Other intervention5 days of strict bedrest. Participants are allowed to sit up in bed, but will perform any bathing or bathroom activities in a wheelchair.
Treatment groups
12 Participants
are divided into 1 treatment groupGroup A: Experimental: BedrestExperimental treatment 1 intervention
Trial outcomes
Primary outcomes
1
Quadriceps muscle volume
Quadriceps muscle volume will be assessed by magnetic resonance imaging.
Time frame
Day 0, and 5Secondary outcomes
1
Muscle strength
Knee extensor peak torque will be assessed using dynamometry
Time frame
Day -14, -9, and 52
Knee extensor power
Peak isotonic power determined at 30% maximal isometric voluntary contraction torque using a dynamometer
Time frame
Day -14, -9, and 53
Muscle fatiguability
Knee extensor fatiguability will be determined by the decrease in isotonic power across 40 contractions at 30% MVC using dynamometry.
Time frame
Day -14, -9, and 54
Muscle cross-sectional area
Change in quadriceps muscle cross sectional area measured by magnetic resonance imaging scan.
Time frame
Day 0 and 5Sponsors and contacts
Click on the lead sponsor to view all of their trials.
Queen's University
Lead sponsor
Kingston Health Sciences Centre
Sponsor institution
Canadian Institutes of Health Research (CIHR)
Collaborator
Source ClinicalTrials.gov