Health TrialsbyHealthPanel

Immunoprofilling of Peripheral Blood Mononuclear Cells in Children With Attention Deficit/Hyperactivity Disorder

ConditionsADHDImmune SystemClinical Syndrome
Trial statusRecruiting
Trial phaseNot listed
Trial typeObservational
Biological sexAll
Age6-12
SponsorFirst Affiliated Hospital of Zhejiang University

About this trial

The goal of this observational study is to learn about The immune cell landscape of peripheral blood mononuclear cells in children with ADHD compared to typically developing controls. The main question it aims to answer are: 1.Testing the differences in immune cell subpopulations, protein expression and signaling pathways and cell subsets between two groups 2. Exploring the correlations between immune function in PBMC and resting-state brain functional networks in children with ADHD.

Participants will be taken peripheral blood about 5 ml , cognitive assessment including Intelligence testing, Stroop color-word test and Trail making test, clinical interview and brain structural and functional MRI.

Eligibility criteria

This trial accepts healthy volunteers

Qualifiers

None

Disqualifiers

None

Trial population

Children and teenagers ages 6-12 years

Trial design

Design model

Case-control

Time perspective

Prospective

Treatments tested in this trial

  • drow blood

    Diagnostic test

    Participants will be taken peripheral blood about 5 ml

Treatment groups

100 Participants
are divided into 2 treatment groups
Group A: ADHD children1 intervention
Group B: typically developing controls1 intervention

Trial outcomes

Primary outcomes

1

the differences in immune cell subpopulations, protein expression , signaling pathways between ADHD and typically developing children

Blood samples of ADHD patients and normal control groups were collected, peripheral blood mononuclear cells were extracted, and 41 protein markers on cell surface were detected and analyzed by CyTOF technology, and detailed atlas of ADHD immune cells were drawn for immunological index analysis. These indicators include cytokine levels, immune cell subsets, and immune-related gene expression.

Time frame
2023.11.1-2023.12.31 Participant recruitment and deta collections
2

Clinical assessment and cognitive function testing

The Clinical Diagnostic Interview Scale (CDIS) was used to evaluate ADHD types, comorbidities, types, comorbidities and clinical symptoms by child psychiatrists with the title of attending psychiatrist or above. ADHD-RS symptom assessment questionnaire was used to assess the severity of symptoms. Cognitive function tests include child IQ, Stroop color word naming test, and Trail making test (TMT).

Time frame
2023.11.1-2023.12.31 Participant recruitment and deta collections
3

Brain MRI structure and resting state data

High-resolution brain structure images were acquired using 3D Magnetization Prepared Rapid Acquisition Gradient-echo (MPRAGE). The FMRI images were acquired by spin-echo-echo planar imaging (SE-EPI)

Time frame
2023.11.1-2023.12.31 Participant recruitment and deta collections

Secondary outcomes

1

General and demographic data collection

A comprehensive questionnaire included name, age, sex, height, weight, age of first onset, course of disease, treatment, family history, past history, personal history

Time frame
2023.11.1-2023.12.31 data collection

Other outcomes

Sponsors and contacts

Click on the lead sponsor to view all of their trials.

First Affiliated Hospital of Zhejiang University

Lead sponsor

Source ClinicalTrials.gov
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