Impact of Diet-induced Change in Energy Balance on Metabolism in Endurance Athletes

Trial statusNot yet recruiting
Trial phaseNot applicable
Trial typeInterventional
Biological sexAll
Age18-49
SponsorUniversity of Bath

About this trial

Recent research has suggested that increasing levels of physical activity are associated with a reduction in the independent components that contribute to total energy expenditure (such as resting metabolic rate and non-exercise movement) - this occurs to conserve energy required for physical activity where energy provision becomes scarce. There are potential deleterious health and performance consequences of a reduced energy supply to fundamental metabolic processes, putting individuals regularly undertaking high levels of physical activity, such as endurance athletes, at risk. However, this association is largely based on observational data in only moderately active populations, and it is currently unclear what role energy balance status and biological sex has on this relationship.

This research intends to address these unknowns by assessing the impact of diet-induced manipulation of energy balance (conditions of energy deficit and energy surplus) in individuals undertaking habitually high levels of physical activity on independent components of total energy expenditure (resting metabolism, exercise and non-exercise movement).

Male and female athletes conducting regular moderate-to-high training volumes will undertake a randomised crossover study with a 7-day state of energy deficit and a 7-day state of energy surplus. Participants will continue to live and train as normal, but their diet will be controlled by specific food provision over the intervention periods in order to facilitate both conditions. Independent components of energy expenditure, markers of health, metabolism and performance will be measured to allow for comparison of conditions.

Eligibility criteria

This trial accepts healthy volunteers

Qualifiers

Self-identified endurance-trained sport participants

Training volume: >7 hours per week endurance training

Training frequency: at least 5 days per week

Disqualifiers

Diagnosis of Relative Energy Deficiency in Sport (REDs)

Active eating disorder (EDE-Q)

Active flare of a chronic disease (e.g. inflammatory bowel disease)

Type 1 or 2 diabetes mellitus

Trial design

Design model

Crossover

Treatments tested in this trial

  • Controlled energy-deficit diet

    Dietary supplement

    Participants receive a prepared diet providing approximately 50% of their estimated daily energy expenditure to induce a sustained energy deficit

  • Habitual diet with surplus snacks

    Dietary supplement

    Participants continue their normal diet with the addition of high-calorie snack items to achieve an approximate daily energy surplus

Treatment groups

20 Participants
are divided into 2 treatment groups
Group A: Energy deficitExperimental treatment 1 intervention
Group B: Energy surplusExperimental treatment 1 intervention

Trial outcomes

Primary outcomes

1

Resting metabolic rate (RMR) in kcal/day

The effect of a 7-day period of energy expenditure-matched diet-induced energy deficit versus energy surplus on RMR. Measured via indirect calorimetry using the Douglas bag method. Expired gas will be collected in a seated, fasted state under thermoneutral conditions, and oxygen consumption and carbon dioxide production will be used to calculate energy expenditure.

Time frame
Measured at lab visits 1-5 (baseline and pre- and post-interventions) from 0 to 12 weeks.

Secondary outcomes

1

Total energy expenditure (from doubly labelled water) in kcal/day

Time frame
Measured during both 7-day interventions starting at approximately week 4 and week 9.
2

Total energy expenditure ( from sum of independent components of energy expenditure) in kcal/day

Time frame
Measured during both 7-day interventions starting at approximately week 4 and week 9
3

Peripheral blood mononuclear cell (PBMC) mitochondrial respiration

Assessment of mitochondrial respiratory function in isolated PBMCs using high-resolution respirometry (Oroboros). Measures include basal (routine), leak, oxidative phosphorylation (OXPHOS), and electron transfer system (ETS) capacity states. Data will be used to assess changes in mitochondrial function in response to exercise and nutritional intervention. Units: pmol O₂·s-¹·10⁶ cells-¹ (picomoles of oxygen consumed per second per million PBMCs).

Time frame
Measured pre- and post-exercise at visits 2/3 (pre- and post-intervention 1) and visits 4/5 (pre- and post-intervention 2), approximately weeks 4-12.
4

Sub-maximal exercise performance (during steady-state treadmill exercise)

Running economy (oxygen consumption at a fixed submaximal speed, expressed as ml O₂·kg-¹·min-¹)

Time frame
Measured at lab visits 1-5 (baseline and pre- and post-interventions) from 0 to 12 weeks.

Other outcomes

Sponsors and contacts

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University of Bath

Lead sponsor

Royal Centre for Defence Medicine

Collaborator

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