About this trial
Recent research has suggested that increasing levels of physical activity are associated with a reduction in the independent components that contribute to total energy expenditure (such as resting metabolic rate and non-exercise movement) - this occurs to conserve energy required for physical activity where energy provision becomes scarce. There are potential deleterious health and performance consequences of a reduced energy supply to fundamental metabolic processes, putting individuals regularly undertaking high levels of physical activity, such as endurance athletes, at risk. However, this association is largely based on observational data in only moderately active populations, and it is currently unclear what role energy balance status and biological sex has on this relationship.
This research intends to address these unknowns by assessing the impact of diet-induced manipulation of energy balance (conditions of energy deficit and energy surplus) in individuals undertaking habitually high levels of physical activity on independent components of total energy expenditure (resting metabolism, exercise and non-exercise movement).
Male and female athletes conducting regular moderate-to-high training volumes will undertake a randomised crossover study with a 7-day state of energy deficit and a 7-day state of energy surplus. Participants will continue to live and train as normal, but their diet will be controlled by specific food provision over the intervention periods in order to facilitate both conditions. Independent components of energy expenditure, markers of health, metabolism and performance will be measured to allow for comparison of conditions.
Eligibility criteria
This trial accepts healthy volunteersQualifiers
Self-identified endurance-trained sport participants
Training volume: >7 hours per week endurance training
Training frequency: at least 5 days per week
Disqualifiers
Diagnosis of Relative Energy Deficiency in Sport (REDs)
Active eating disorder (EDE-Q)
Active flare of a chronic disease (e.g. inflammatory bowel disease)
Type 1 or 2 diabetes mellitus
Trial design
Crossover
Treatments tested in this trial
Controlled energy-deficit diet
Dietary supplementParticipants receive a prepared diet providing approximately 50% of their estimated daily energy expenditure to induce a sustained energy deficit
Habitual diet with surplus snacks
Dietary supplementParticipants continue their normal diet with the addition of high-calorie snack items to achieve an approximate daily energy surplus
Treatment groups
Trial outcomes
Primary outcomes
Resting metabolic rate (RMR) in kcal/day
The effect of a 7-day period of energy expenditure-matched diet-induced energy deficit versus energy surplus on RMR. Measured via indirect calorimetry using the Douglas bag method. Expired gas will be collected in a seated, fasted state under thermoneutral conditions, and oxygen consumption and carbon dioxide production will be used to calculate energy expenditure.
Secondary outcomes
Total energy expenditure (from doubly labelled water) in kcal/day
Total energy expenditure ( from sum of independent components of energy expenditure) in kcal/day
Peripheral blood mononuclear cell (PBMC) mitochondrial respiration
Assessment of mitochondrial respiratory function in isolated PBMCs using high-resolution respirometry (Oroboros). Measures include basal (routine), leak, oxidative phosphorylation (OXPHOS), and electron transfer system (ETS) capacity states. Data will be used to assess changes in mitochondrial function in response to exercise and nutritional intervention. Units: pmol O₂·s-¹·10⁶ cells-¹ (picomoles of oxygen consumed per second per million PBMCs).
Sub-maximal exercise performance (during steady-state treadmill exercise)
Running economy (oxygen consumption at a fixed submaximal speed, expressed as ml O₂·kg-¹·min-¹)
Sponsors and contacts
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University of Bath
Lead sponsor
Royal Centre for Defence Medicine
Collaborator
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