About this trial

This study will compare mitochondrial function and leg blood flow during exercise in healthy endurance-trained females and males aged 19-35.

Eligibility criteria

This trial accepts healthy volunteers

Qualifiers

can understand and communicate in English;

are 19 to 35 years old;

answer "No" to all questions on the Canadian Society for Exercise Physiology Get Active Questionnaire (CSEP-GAQ), or have approval from a doctor to do maximal exercise testing;

are healthy enough to do hard exercise;

Disqualifiers

• currently use cigarettes, vaping products, or other nicotine products, or have used them in the past 2 years;

have a major uncontrolled heart, blood vessel, muscle, metabolic, or nervous system condition

have a medical condition that makes maximal exercise unsafe for you

have heart or lung disease, or a condition that affects your heart, lungs, blood vessels, or muscles

Trial design

Design model

Parallel

Treatments tested in this trial

Exercise
Other intervention
Exercise - Men
Exercise
Other intervention
Exercise - Females

Treatment groups

24 Participants

are divided into 2 treatment groups

Group A: Healthy MalesActive comparator 1 intervention

Group B: Healthy FemalesActive comparator 1 intervention

Trial outcomes

Primary outcomes

1

Isolated mitochondria maximal state 3 respiration (pmols O2/microgram) measured via an ouroborous oxygraph (mitochondrial respirometry)

This measure quantifies the maximal rate of oxygen consumption (picomoles O2/ micogram of mitochondrial protein) when ADP, Malate, Succinate, Pyruvate and Glutamate have all been addded to the chamber.

Time frame

Baseline

2

Maximal state 3 respiration in permeabilized Fibers (pmols O2/mg) measured via an ouroborous oxygraph (mitochondrial respirometry)

This measure quantifies the maximal rate of oxygen consumption (picomoles O2/ milligram of muscle tissue) when ADP, Malate, Succinate, Pyruvate and Glutamate have all been added to the chamber.

Time frame

Baseline

3

Fiber type 1 specific maximal state 3 respiration (pmols O2/mg) measured via an ouroborous oxygraph (mitochondrial respirometry)

This measure quantifies the maximal rate of oxygen consumption (picomoles O2/ micogram of mitochondrial protein) when ADP, Malate, Succinate, Pyruvate and Glutamate have all been added to the chamber.

Time frame

Baseline

4

Fiber type 2 specific maximal state 3 respiration (pmols O2/mg) measured via an ouroborous oxygraph (mitochondrial respirometry)

This measure quantifies the maximal rate of oxygen consumption (picomoles O2/ micogram of mitochondrial protein) when ADP, Malate, Succinate, Pyruvate and Glutamate have all been addded to the chamber.

Time frame

Baseline

Investigating Sex Differences in Mitochondrial Function and Leg Blood Flow During Exercise

About this trial

Eligibility criteria

Qualifiers

Disqualifiers

Trial design

Treatments tested in this trial

Treatment groups

Trial outcomes

Primary outcomes

Secondary outcomes

Other outcomes

Sponsors and contacts