Long-term Adaptations of Skeletal Muscle After Hybrid Training
ConditionObesity & Overweight
Trial statusRecruiting
Trial phaseNot applicable
Trial typeInterventional
Biological sexAll
Age30-50
SponsorUniversity of Thessaly
About this trial
Obesity is a major challenge for public health and renders it imperative to reduce its prevalence. High intensity interval training (HIIT) is a form of exercise training that can efficiently induce weight loss in adults with overweight or obesity, even in the absence of dietary intake manipulation. Hybrid type training represents a form of HIIT, that incorporates both cardiorespiratory and musculoskeletal stimuli, by combining multiple types of exercise into a circuit-type, interval style workout. Recent evidence suggests that long-term participation in hybrid HIIT results in significant health-related benefits. However, the molecular mechanisms driving the chronic effects of hybrid HIIT on cardiometabolic and musculoskeletal health remains to be elucidated.
Eligibility criteria
This trial accepts healthy volunteersQualifiers
BMI: >25 kg/m2 and <40 kg/m2
Untrained individuals (abstain >1 year from exercise training)
No dietary intervention over the last 6 months preceding the study
Low cardiorespiratory fitness level (VO2max: <45 ml/kg/min)
Disqualifiers
Low participation rate (<80% completion of the exercise training sessions)
Unbalanced diet
Participation in additional exercise training regimes
Cosumption of anti-inflammatory of pain relief medication
Trial design
Design model
Parallel
Treatments tested in this trial
Exercise training
Other interventionParticipants will perform a six-month hybrid training program while receiving a balanced diet. The periodization of hybrid training intervention will consist of three 2-month phases of gradually increased exercise intensity and volume. In every training will participate 5-8 individuals. The training will contain 6-12 different exercises (stations), depending on the phase of the intervention, which will be executed in a circuit for a total of 2-3 rounds, with 2-3 minutes of rest period between sets (depending on the phase). The exercise execution will last 20-45 seconds, and the rest between them will last 30-60 seconds (depending on the phase), while the exercise intensity will range from 75 to 85% of maximal heart rate. The stations of hybrid training will contain multi-joint exercises or neuromuscular activation exercises using either body weight resistance or portable equipment.
Control group
Other interventionParticipants will receive a balanced diet but will not participate in any type of exercise training over a six month period.
Treatment groups
30 Participants
are divided into 2 treatment groupsGroup A: Intervention groupExperimental treatment 1 intervention
Group B: Control groupActive comparator 1 intervention
Trial outcomes
Primary outcomes
1
Change in mitochondrial size
Mitochondrial size will be measured using transmission electron microscope
Time frame
At baseline and at 6 months2
Change in mitochondrial density
Mitochondrial density will be measured using transmission electron microscope
Time frame
At baseline and at 6 months3
Change in mitochondrial count
Mitochondrial count will be determined using transmission electron microscope
Time frame
At baseline and at 6 months4
Change in mitochondrial distribution
Mitochondrial distribution will be determined using transmission electron microscope
Time frame
At baseline and at 6 monthsSecondary outcomes
1
Change in density and distribution of capillaries
Capillarization will be determined using immunohistochemistry techniques
Time frame
At baseline and at 6 months2
Change in skeletal muscle fiber typing
Fiber typing will be determined using immunohistochemistry techniques
Time frame
At baseline and at 6 months3
Change in GLUT-4 protein expression
GLUT-4 protein expression will be assessed using immunoblotting techniques
Time frame
At baseline and at 6 months4
Change reduced glutathione content in skeletal muscle cells
Reduced glutathione content will be determined spectrophotometrically
Time frame
At baseline and at 6 monthsSponsors and contacts
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Source ClinicalTrials.gov