About this trial
Poor sleep quality is very common in modern society. Walnuts contain many nutrients that may be helpful for sleep, including melatonin and polyphenols. Some studies show that eating foods high in melatonin and polyphenols improves sleep quality, but walnuts have not been studied specifically. This study proposes to test if eating walnuts improves sleep compared to a food that lacks these sleep-promoting factors. The investigators expect that walnut consumption for 4 days will increase melatonin levels and lead to better sleep quality compared to a high-carbohydrate, high-sugar food. The study will enroll middle-aged and older adults with sleep complaints to participate in this study. Each person will eat the two different foods for 4 days each in random order. The 4-day periods will be separated by at least 2-3 weeks. Sleep quality will be measured by questionnaire and with a wrist monitor every day. The investigators will also do a sleep study using electroencephalography (EEG) on night 3 and take measures of circadian physiology (natural body rhythms) in the laboratory on day 4 (including overnight) by measuring body temperature and blood and urine melatonin. The study findings may provide new options to improve sleep health from increased walnut consumption.
Eligibility criteria
This trial accepts healthy volunteersQualifiers
Equal numbers of men and women (12 male and 12 post-menopausal female)
Equal number of individuals with normal weight (18.5-24.9 kg/m2) and overweight (25-29.9 kg/m2)
Participants will self-report poor sleep quality, reflected by a global score >5 on Pittsburgh Sleep Quality Index
Disqualifiers
Diagnosed sleep disorder
smoking, excessive caffeine intake (>300 mg/day)
shift work
chronic pain
Trial design
Crossover
Treatments tested in this trial
Walnut consumption
Other interventionParticipants will add one serving (1 oz) of walnuts at their self-defined breakfast, lunch, and dinner for 4 days. The study will provide study foods at 3 main meals each day to evaluate a temporal effect of the food on melatonin concentrations throughout the day.
HCHS consumption
Other interventionParticipants will add one HCHS food (one PopTarts® pastry) to each of their 3 main meals of the day for 4 days. The study will provide study foods at 3 main meals each day to evaluate a temporal effect of the food on melatonin concentrations throughout the day. An energy-matched high-carbohydrate, high-sugar (HCHS) alternative, representative of a common US snack food, on sleep quality in adults with habitually poor sleep quality.
Treatment groups
Trial outcomes
Primary outcomes
Serum melatonin
Serum melatonin will be measured via last testing of blood samples collected on day 4, in an inpatient setting. Repeated for each phase.
Sleep quality index
Sleep quality will be assessed daily using wrist actigraphy. Variables include sleep fragmentation index and sleep efficiency. Repeated for each phase.
Slow wave sleep index
Slow wave sleep will be measured using electroencephalography, in the participant's home. Repeated for each phase.
Secondary outcomes
6-sulfatoxymelatonin level
This is to measure Melatonin production in urine samples. Repeated for each phase.
Body temperature
Proximal to distal body temperature gradient measured using thermochron devices (iButtons). Repeated for each phase. In patient. Over 24 hours.
Sleep quality score
Self-reported sleep quality assessed using the Karolinska Sleep Diary (higher score represents better sleep). Repeated for each phase.
Other outcomes
NFkB level
This is to measure Endothelial cell inflammation in endothelial cells collected from a forearm vein. Repeated for each phase. Fasted sample.
Sponsors and contacts
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Columbia University
Lead sponsor
United States Department of Agriculture (USDA)
Collaborator
California Walnut Commission
Collaborator