The goal of this observational study was to learn about metabolomics profiles in vitreoretinal diseases by nuclear magnetic resonance (NMR)using vitreous fluid. The main question it aimed to answer was abnormal biomarkers for common retinal diseases such as idiopathic macular hole(IMH), diabetic retinopathy(DR) and retinal detachment(RD). Participants would not be subjected to any intervention and the investigators would only collect preoperative information and remaining vitreous samples. The investigators divided the participants into groups with appropriate disease names based on the disease diagnosis, such as IMH group, DR group, and RD group. The MH group was used as a control group, investigators compared other groups to see the metabolomic abnormalities.
Confirmation of IMH diagnosis with concomitant need for PPV surgery;
Confirmation of DR diagnosis with concomitant need for PPV surgery;
Confirmation of RD diagnosis with concomitant need for PPV surgery;
Confirmation of IMM diagnosis with concomitant need for PPV surgery;
Diagnosed vitreoretinal disease with no need for PPV surgery;
Previous history of PPV surgery;
Combination of other active ophthalmic diseases, such as acute conjunctivitis and uveitis;
Combination of serious systemic diseases such as hypertension and diabetes mellitus.
The enrolled participants were patients who attended Renmin Hospital of Wuhan University with a confirmed diagnosis of vitreoretinal disease. As a result, most of the participants were residents of Hubei Province, China. It is expected that vitreous fluid samples collected from patients with IMH, DR, RD, IMM, and RVO were 50, 100, 100, 50, and 50, respectively, for a total of 450, with about a mean age of 56 years.
Case-control
Prospective
Not listed
Relative amino acid concentration(Unite: %)
NMR provides spectral data to show the relative concentration of various amino acids, and the use of NMR to measure vitreous humor and compare changes in amino acid content across disease species leads to speculation about biological markers of disease progression.
Relative concentrations of macromolecules such as glucose and lipids(Unite: %)
Macromolecules such as glucose and lipids also play a role in the metabolism of vitreoretinal diseases, so NMR spectroscopy will be used to show the relative concentration of macromolecules.
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