MORPHEE : Mechanisms of Cell Death Induced by Extracorporeal Photochemotherapy
Trial statusRecruiting
Trial phaseNot applicable
Trial typeInterventional
Biological sexAll
Age18-85
SponsorCentre Hospitalier Universitaire de Besancon
About this trial
The objective of this study is to describe the type of cell death induced by extracorporeal photochemotherapy, depending on the cell type, using a panel of complementary analysis techniques.
Eligibility criteria
This trial does not accept healthy volunteersQualifiers
Adult patients
Treated with ECP for at least 1 month, for control of GVHD in hematopoietic cell allograft (acute or chronic GVHD), treatment and control of cellular or humoral rejection in solid organ transplantation (heart, lung, kidney) or in the treatment of cutaneous T-cell lymphoma
Disqualifiers
Subjects with limited legal capacity.
Subjects judged by the investigator to be unlikely to comply with study procedures
Subjects with no social security coverage.
Pregnant women.
Trial design
Design model
Single group
Treatments tested in this trial
Samples collection
Other interventionSampling of 2 samples on the extracorporeal photochemotherapy circuit, before and after sensitization with 8-MOP and UVA irradiation (without additional puncture)
Treatment groups
20 Participants
are divided into 1 treatment groupGroup A: Patients undergoing extracorporeal photochemotherapyOther 1 intervention
Trial outcomes
Primary outcomes
1
Type of cell death induced by 8-MOP sensitization and UVA irradiation
Caspase-3 activation in Western blot
Time frame
24 hours2
Type of cell death induced by 8-MOP sensitization and UVA irradiation
Gasdermin D cleavage in Western blot
Time frame
24 hours3
Type of cell death induced by 8-MOP sensitization and UVA irradiation
MLKL (mixed lineage kinase domain-like pseudokinase) phosphorylation in Western blot
Time frame
24 hoursSponsors and contacts
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Source ClinicalTrials.gov