A significant variation in the serum concentration of the circulating cytokine TWEAK is associated with the onset of an inflammatory attack of MS. Study the concentration variations of the serum soluble form of cytokine TWEAK during the first year of MS and to analyze their correlation with the occurrence of an inflammatory disease outbreak.
a first episode of inflammation and demyelination of the central nervous system affecting the optic nerve, the spinal cord, one cerebral hemisphere, or the brainstem; or confirmed multiple sclerosis
no differential diagnosis at enrollment based on clinical examination and laboratory tests
compliance with the revised 2017 Mac Donald diagnostic criteria
age <18 years old
person in an emergency situation
adult who is subject to a legal protection measure (an adult under guardianship, conservatorship, or judicial protection), or who is unable to give consent
not Enrolled in a Social Security Program
Single group
Determination of the circulating serum concentration of cytoquine TWEAK
Diagnostic testBlood samples (20 ml of blood on dry tube) by venipuncture at the bend of the elbow will be made at T0, then monthly for a period of 1 year . The sera will be isolated by centrifugation and then frozen at -80 degrees Assays of the soluble form of TWEAK and TNF will be performed by ELISA blot technique developed by the team.
The serum concentration of soluble TWEAK
We will then compare the soluble TWEAK concentrations observed in patients with anti-TWEAK antibodies and in patients without anti-TWEAK antibodies. We will study the correlation between the concentrations of soluble TWEAK and those of soluble TNF.
The titers of anti-TWEAK antibodies
Measured via blood samples taken at baseline and then 3 to 6 times thereafter, with at least 1 month between each sample
Functional properties of anti-TWEAK antibodies
Positive or negative test Measured via blood samples taken at baseline and then 3 to 6 times thereafter, with at least 1 month between each sample
Soluble TWEAK concentrations
Measured via blood samples taken at baseline and then 3 to 6 times thereafter, with at least 1 month between each sample
Serum concentrations of soluble TNF
Measured via blood samples taken at baseline and then 3 to 6 times thereafter, with at least 1 month between each sample
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