Twenty patients with centronuclear myopathy and twenty age- and sex-matched, muscle-healthy controls will undergo diagnostic examination. Study participants will undergo physical examination, clinical and functional testing, and multispectral optoacoustic tomography (MSOT) scanning at predefined muscle sites (paraspinal muscles, trapezius muscle, deltoid muscle, forearm flexors, quadriceps muscle, adductor muscles, ischiocrural muscles, triceps surae muscle, and tibialis anterior).
None
Pregnancy
Nursing mothers
Tattoo in the area of the examination field
Subcutaneous fatty tissue > 3 cm
n= 20 CNM n = 20 HV
Cohort
Cross-sectional
MSOT
Diagnostic testMultispectral optoacoustic tomography (MSOT) enables the detection of specific endogenous chromophores such as collagen, myoglobin or haemoglobin using a non-invasive approach comparable to conventional ultrasound. Instead of sound waves, MSOT illuminates the tissue with near-infrared light of transient energy, which is absorbed and leads to thermoelastic expansion of certain molecules. This expansion generates ultrasound waves that are detected by the same device. The multispectral illumination and unmixing then enable the precise localization and quantification of muscle-specific subcellular structures. MSOT has already shown the potential to visualize the muscle structure and clinical extent of muscle disease in patients with Duchenne muscular dystrophy, spinal muscular atrophy and the late-onset Pompe disease (LOPD) and to distinguish these patients from healthy volunteers. To date, there is no optoacoustic data on CNM.
Optoacoustic spectrum of MSOT Imaging (all in arbitrary units)
Comparison of optoacoustic spectrum from patients with diagnosed CNM and their age- and sex matched Healthy Volunteers
Comparison of MSOT-Derived Hemoglobin and Myoglobin Signals
Quantitative comparison of MSOT-derived hemoglobin and myoglobin signals (arbitrary units).
Comparison of Oxygenated vs. Deoxygenated Hemoglobin Signal
Quantitative comparison of oxygenated and deoxygenated hemoglobin signals.
Comparison of Oxygenated Hemoglobin and Lipid Signal
Quantitative comparison of oxygenated hemoglobin and lipid signals.
Comparison of deoxygenated Hemoglobin and Lipid Signal
Quantitative comparison of deoxygenated hemoglobin and lipid signals.
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