Nanopore Sequencing in Ophthalmology
ConditionEndophthalmitis
Trial statusRecruiting
Trial phaseNot listed
Trial typeObservational
Biological sexAll
Age18-99
SponsorMedical University of Graz
About this trial
In this pilot study, the feasibility of the Oxford Nanopore MinION to identify pathogens from vitrectomy samples in eyes with endophthalmitis shall be assessed. The MinION is a low cost commercially available device for DNA/ RNA analysis that, in studies, has been used for pathogen determination in various infectious diseases as well as for the genetic characterization of hematologic tumors.
Eligibility criteria
This trial does not accept healthy volunteersQualifiers
Eyes of patients with endophthalmitis
Disqualifiers
No informed consent possible
Participation in other studies
Trial population
Eyes of adult patients with endophthalmitis
Trial design
Design model
Case-only
Time perspective
Prospective
Treatments tested in this trial
Not listed
Trial groups
No trial groups listed
Trial outcomes
Primary outcomes
1
Pathogen detection
Pathogens detected by Nanopore sequencing versus microbiologic culture
Time frame
In vitro analysis after vitrectomy for endophthalmitis. Start of analysis: Within the next working day after sample acquisition. Time for Nanopore sequencing: Approximately up to 10 hours. Time for culture: 24 hours up to 14 days.Secondary outcomes
1
Time to pathogen detection
Time in days from gathering of the sample until arrival of results
Time frame
Time measured from start of analysis until arrival of results. Time for Nanopore sequencing: Approximately up to 10 hours. Time for culture: 24 hours up to 14 days.2
Visual acuity
Visual acuity measured with Snellen charts
Time frame
Gathered from patient charts two months after vitrectomy.Sponsors and contacts
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Source ClinicalTrials.gov