Effect of Melatonin Administration on Systemic Lupus Erythematosus Patients

Serum Interleukin- 6 (IL-6)

Blood samples will be drawn from the patients at baseline and after 12 weeks of intervention. Blood samples will be collected at baseline, centrifuged and stored as serum at - 80°C or -20°C until analysis. Enzyme-linked immunosorbent assay (ELISA) method will be used to measure Interleukin 6 concentrations

SLEDAI -2K Score

A)Items: It includes 24 items, with 16 clinical and 8 laboratory-based descriptors. B)Domains: The items are grouped into nine organ systems: central nervous system, vascular, musculoskeletal, serosal, dermal, constitutional, renal, immunological, and hematological. C)Scoring: Each item is scored as present or absent, typically based on the preceding 30 days. D)Total Score: The individual item scores are summed to produce a global score, ranging from 0 to 105.

Serum SOD

Superoxide dismutase (SOD) measured by Spectrophotometric Method, by tracking the inhibition of superoxide radical-driven reactions, often involving the reduction of nitro blue tetrazolium (NBT) or the autoxidation of other compounds like BXT-01050, which produce a color change that is measured at a specific wavelength, typically around 525 nm or 560-562 nm. The rate of this color change in the presence of a sample is compared to the rate in a blank, with the percent inhibition of the reaction directly correlating to the amount of SOD present in the sample.

Arabic version form of Systemic Lupus Erythematosus-Specific Quality of Life Questionnaire (SLEQOL-Arabic Version)

The questionnaire consists of 40 items covering multiple domains relevant to SLE. These items are grouped into 6 domains which are: 1. Physical functioning / mobility 2. Role limitation / daily activities 3. Self-image / appearance 4. Emotional / mood 5. Symptoms 6\_Social / interpersonal relations Each of the 40 items is rated using a 7-point Likert scale (1=Not at all affected and 7=Extremely affected). Higher scores generally reflect poorer QoL.

Arabic version of Fatigue Severity Scale (FSS)

FSS is a self-reported questionnaire that is simple and easy to use. It consists of 9 statements that rate the severity of the patient's fatigue symptoms in terms of how these symptoms affect motivation, exercise, physical function, and activities of daily living Reflecting on their condition over the past month, patients score each item from 1 to 7, based on the extent, to which they agree or disagree with each statement (1 = strong disagreement, 7 = strong agreement).