The goal of the project is to determine the protein quality of alternative protein sources, honey chlorella and Faba bean, ingested as mixed meals in healthy subjects equipped with naso-ileal tube. For this purpose, Chlorella and Faba bean are intrinsically labelled with 15N. Faba bean is processed to produce a meat analogue. Chlorella is introduced in a drink. The protein quality is determined by following the digestive and metabolic fate of 15N during a 8h postprandial investigation.
18<BMI<30
Good health (WHO=0)
For woman: use of birth control
food allergy
allergy against latex
pregnant women
Positive serology for AgHBS, AcHbc, HCV, HIV
Parallel
Single meal containing 20 g of 15N intrinsically labelled protein from honey chlorella
Dietary supplementIngestion of a 15N labeled test meal. Subjects are equipped with a nasoileal tube. 15N is followed in biological fluids (plasma, effluents, urines).
Single meal containing 20 g of 15N intrinsically labelled protein from faba bean meat analogue
Dietary supplementIngestion of a 15N labeled test meal. Subjects are equipped with a nasoileal tube. 15N is followed in biological fluids (plasma, effluents, urines).
Ileal digestibility of protein and amino acids
The measurement of nitrogen (mmol) and 15N enrichment (atom %) in ileal samples allow to determine the amount of dietary nitrogen in the lumen. Dietary nitrogen that is recovered in the ileal samples are then considered as non absorbed.
Net Postprandial Protein Utilization (NPPU)
The measurement of urea (mmol) and 15N enrichment of urea (atom %) allow to determine the amount of dietary N that was transferred to urea, thus accounting for metabolic losses. Metabolic losses of dietary N, together with digestive losses of dietary N, are not retained in the body.
Ileal digestibility of amino acids
The measurement of amino acid concentration (mmol) and their individual 15N enrichment (atom %) allow the determination of dietary amino acids remaining in the lumen. Dietary amino acids that are recovered in the ileal samples are then considered as non absorbed.
Nitrogen metabolites in blood in response to the ingestion of 15N fava bean
urea and amino acids (mmol/l)
Plasma glucose in blood in response to the ingestion of 15N fava bean
plasma glucose (mmol/l)
Plasma insulin in blood in response to the ingestion of 15N fava bean
plasma insulin (pmol/l)
Ileal microbiota
Microbial DNA sequencing
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Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
Lead sponsor
Assistance Publique - Hôpitaux de Paris
Sponsor institution
European Commission
Collaborator
