Role of USP35 in the Detection of Ferroptosis in Juvenile Autoimmune Hepatitis

ConditionAutoimmune Hepatitis

Trial statusNot yet recruiting

Trial phaseNot listed

Trial typeObservational

Biological sexAll

Age1-18

SponsorAssiut University

About this trial

1. To evaluate the immunohistochemical expression of USP35 in cases of juvenile autoimmune hepatitis and control cases. 2. To correlate this with the level of necro-inflammation and extent of fibrosis using Massion's trichrome stain in cases of juvenile autoimmune hepatitis.

Eligibility criteria

This trial accepts healthy volunteers

Qualifiers

Patients aged between one day and 18 years (children)

patients diagnosed clinically and physically with juvenile autoimmune hepatitis including seronegative autoimmune hepatitis cases

Disqualifiers

Cases with inadequate tissue in the paraffin block

Children with viral hepatitis

patients above the age of 18 years old

Trial population

Cases with adequate tissue in the paraffin blocks from Laboratories of Surgical Pathology at Assiut University and private labs will be recruited in this study during 5 years period from 2019 to 2024. Approximately 50 cases of juvenile autoimmune hepatitis and 10 control cases will be taken Histological diagnosis of H\&E stained slides will be reviewed and the histological criteria will be assessed.

Trial design

Design model

Other

Time perspective

Cross-sectional

Treatments tested in this trial

Not listed

Trial groups

60 Participants

are grouped into 2 trial groups

Group A: cases with juvenile autoimmune hepatitis

Group B: control group

Trial outcomes

Primary outcomes

Expression of USP35 in cases of juvenile autoimmune hepatitis

Expression of USP35 is an indicator of ferroptosis so the investigators can detect the relationship between ferroptosis and the pathogenesis of juvenile autoimmune hepatitis

Time frame

baseline

Secondary outcomes

To correlate the expression of USP35 with the level of necro-inflammation and extent of fibrosis using Massion's trichrome stain in cases of juvenile autoimmune hepatitis

To know whether ferroptosis is an initiator or mediator in the pathogenesis of juvenile autoimmune hepatitis, the investigators will use Massion's trichrome stain to assess the level of neuroinflammation and extent of fibrosis

Time frame

baseline

Locations

This trial has no locations.

Sponsors and contacts

Click on the lead sponsor to view all of their trials.

Assiut University

Lead sponsor

Source ClinicalTrials.gov

This trial is not recruiting at the moment. You can still explore other options: