The purpose of this research study is to validate (check the accuracy of) laboratory assays, intravenous catheter insertion, and equipment or devices and their reproducibility, which is necessary to perform high quality research on chronic diseases (obesity, pre-diabetes and type-2 diabetes, dyslipidemia, non-alcoholic fatty liver disease, cardiovascular disease, etc.), nutrition, and metabolism (the process by which a substance is handled in your body) at the University of Missouri. As technology changes and we start to use new testing methods, it is necessary to compare results from old tests, equipment and devices and new tests, equipment, or devices and the reproducibility of these measurements to make sure we are getting accurate results. Reproducibility means performing the same test more than once to see if the same results can be achieved each time.
≥18 and ≤75 years of age
body mass index ≥18.5 and ≤45 kg/m2
Weight stable (i.e., ≤3% change)
≤150 min of structured exercise/week for at least 2 months before entering the study
<18 and >75 years of age
body mass index <18.5 or >45 kg/m2
history of or current significant organ system dysfunction (e.g., heart disease, stroke, diabetes, cancer in remission for <5 years, dementia, chronic kidney disease)
allergies or intolerances to meal ingredients, vegans or vegetarians
convenience population of volunteers in mid-Missouri region
Cohort
Prospective
endothelial cell collection
Other interventionParticipants may opt to have testing performed including blood sampling, intravenous catheter placement with endothelial cell collection, oral glucose tolerance test, consuming a test meal, having imaging performed such as MRI, DEXA, MRS to investigate use of different assays, different sample treatment approaches to determine optimal conditions that produce the most accurate and reproducible results, and/or repeat testing on different days or with different equipment that measures the same variable.
Validation and reproducibility testing for blood assays
insulin, C-peptide, glucagon, substrates (free fatty acids, glucose, ketone bodies) and biomarkers of substrate metabolism (e.g., cytokines and adipokines)
Validation and reproducibility testing for endothelial cell collection
endothelial cell quantity collected
Validation and reproducibility testing for mixed meal ingestion
tolerability and palatability of meal type (protein concentration, carbohydrate concentration)
Validation and reproducibility testing for glucose tolerance testing
glucose levels
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Bettina Mittendorfer
Lead sponsor
University of Missouri-Columbia
Sponsor institution
