Multicenter, non-interventional, retrospective/prospective study of a biological nature, on patients affected by head and neck tumors, for which the collection and use of tissue samples is planned for the study of the mutational profile, the transcriptional profile and the proteomic profile.
Age over 18
Patients with squamous cell tumors of the oral cavity, pharynx or larynx
Surgical patients with primary HNSCC tumors, who have not had previous tumors in other sites or adjuvant treatments prior to surgery.
Availability of adequate material (tissue) for the planned analyses
Presence of distant metastases at the time of diagnosis
Previous head and neck cancer
Second cancer undergoing treatment or follow-up for less than 5 years
Patients with head and neck tumors
Other
Cross-sectional
Culture of organoids
Biological/VaccineCulture of organoids prepared from both the primary tumor and the respective relapse, using the material from the prospective part of the study, the response to treatment will be carried out by macroscopic analysis, evaluating the size and number of organoids before and after treatment,
Organoids cultures
Fresh tissue samples collected from surgery at the reference centers will be selected by the pathologist, and stored at 4°C in special media at the reference laboratories. These samples will be evaluated by means of viabilityassays with ATPlite, the ability to form colonies and apoptosis assays (by FACS). For organoids, the response to treatment will be performed by macroscopic analysis, evaluating the size and number of organoids before and after treatment.
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