The Effect of Fasting on Peak Fat Oxidation, Mitochondrial Coupling and Extracellular Matrix
Trial statusRecruiting
Trial phaseNot applicable
Trial typeInterventional
Biological sexAll
Age18-40
SponsorUniversity of Copenhagen
About this trial
The interventional study will recruit four groups (n=10 per group): Young men and women with normal weight (BMI 18-25), and young men and women with obesity (BMI \>30). The study will investigate the effect of 84 hours of fasting on peak fat oxidation, mitochondrial coupling and the extracellular matrix.
Eligibility criteria
This trial accepts healthy volunteersQualifiers
Healthy normal weight men aged [18,40] and BMI [18,25]
Healthy normal weight women [18,40] and BMI [18,25]
Healthy obese men [18,40] and BMI [30,40]
Healthy obese women [18,40] and BMI [30,40]
Disqualifiers
Present or former cardiovascular disease and chronical inflammatory disease
Consumption of medications and supplements that influence substrate use at rest and/or during exercise
Pregnancy
Trial design
Design model
Parallel
Treatments tested in this trial
Diet
Other interventionLong-term fasting (84 hours), physiological testing before and after fasting: sampling of blood, abdominal adipose tissue and skeletal muscle for molecular analysis and respiratory measurements at rest.
Treatment groups
40 Participants
are divided into 4 treatment groupsGroup A: Young normal weight menExperimental treatment 1 intervention
Group B: Young normal weight womenExperimental treatment 1 intervention
Group C: Young obese menExperimental treatment 1 intervention
Group D: Young obese womenExperimental treatment 1 intervention
Trial outcomes
Primary outcomes
1
The effect of fasting and obesity on protein glycosylation of the extracellular matrix in adipose tissue
The composition and protein glycosylation of the extracellular matrix will be measured in abdominal subcutaneous adipose tissue samples in 20 healthy normal weight men and women and 20 healthy obese men and women.
Time frame
The fasting will last approximately 84 hours.Secondary outcomes
1
The effect of fasting and obesity on peak fat oxidation
40 participants (20 healthy normal weight men and women and 20 healthy obese men and women) will fast for 84 hours. Before and after the fast, peak fat oxidation (mg fat/min) is measured in an incremental bike test using indirect calorimetry.
Time frame
The fasting will last approximately 84 hoursOther outcomes
1
The effect of fasting and obesity on adipose tissue lipolytic capacity, muscle ketone oxidative capacity and mitochondrial coupling.
The lipolytic capacity will be measured in abdominal subcutaneous adipose tissue samples and the capacity for ketone oxidation in vastus lateralis muscle in 20 healthy normal weight men and women and 20 healthy obese men and women.and In addition mitochondrial respiratory capacity will be measured in the muscle and adipose biopsy samples using high-resolution respirometry (Oxygraphs, Oroboros).
Time frame
The fasting will last approximately 84 hours.Sponsors and contacts
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Source ClinicalTrials.gov