This study aims to evaluate the changes in salivary pH and Streptococcus mutans count by preparing a solution containing these sweeteners dissolved in distilled water and administering it to the participants. Saliva samples will be collected before using the mouth rinse, and salivary pH will be measured using a pH meter, while S. mutans count will be analyzed in the laboratory.
After consuming the solution, salivary pH will be assessed at 1 minute, 15 minutes, and 30 minutes, while the growth of S. mutans will be examined 30 minutes post-rinse.
No active carious lesions or oral diseases.
Generally healthy individuals without systemic illnesses.
No prior use of preventive dental treatments
Participants currently undergoing dental treatment.
Those taking medications that alter saliva flow.
Presence of painful oral conditions or systemic diseases. Participants have active caries
Parallel
Monk fruit sweetener mouth rinse
Dietary supplementmonk fruit mouth rinse 10 grams of sweetener powder and 90 ml of distilled water for 30 participants for 1 minute
Splenda (sucralose) sweetener mouth rinse
Dietary supplement10 g of splenda powder with 90 ml of distilled water for 30 participants for 1 minute
Sucrose mouth rinse
Dietary supplement10 g of sucrose powder dissolved in 90 ml of distilled water as a positive control group for 30 participants for 1 minute
salivary ph measurement
the salivary ph will measure quantitatively using a ph meter
Streptococcus mutans count measurement
streptococcus mutans count before mouth rinsing ,and after 30 minutes to evaluate the count if it increase or decrease
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