The Role of Macrophage Activation in Lung Injury Following Ozone Exposure
ConditionAir Pollution
Trial statusRecruiting
Trial phaseNot applicable
Trial typeInterventional
Biological sexAll
Age18-40
SponsorRutgers, The State University of New Jersey
About this trial
The purpose of the study is to better understand the mechanisms of lung injury from ozone exposure. Subjects will participate in two exposure sessions: filtered air and 0.2 ppm ozone. The exposure visits will be at least 2 weeks apart. Subjects will be asked to produce sputum through coughing after each exposure. The samples will be analyzed for macrophage activity.
Eligibility criteria
This trial accepts healthy volunteersQualifiers
None
Disqualifiers
Cardiovascular disease
Respiratory disease
Recent (within 4 weeks) respiratory or COVID-19 symptoms
Diabetes
Trial design
Design model
Crossover
Treatments tested in this trial
Clean Air
Other interventionSubjects will be exposed to clean air for 3 hours.
Ozone
Other interventionSubjects will be exposed to 0.2 ppm ozone for 3 hours.
Treatment groups
120 Participants
are divided into 2 treatment groupsGroup A: Clean Air, Then OzoneExperimental treatment 2 interventions
Group B: Ozone, Then Clean AirExperimental treatment 2 interventions
Trial outcomes
Primary outcomes
1
Metabolic activity of inflammatory cells in induced sputum
The metabolic activity of inflammatory cells in sputum will be assessed by the measurement of ATP production. The measurement will be made on an Agilent Seahorse using a Mitoplate Assay.
Time frame
Within 3 days of exposureSponsors and contacts
Click on the lead sponsor to view all of their trials.
Rutgers, The State University of New Jersey
Lead sponsor
National Institute of Environmental Health Sciences (NIEHS)
Collaborator
Source ClinicalTrials.gov