Antimicrobial Efficacy of Nano-Based ICM on E.F
Trial statusRecruiting
Trial phaseNot applicable
Trial typeInterventional
Biological sexAll
Age19-60
SponsorTanta University
About this trial
This in-vivo study aims to evaluate the antimicrobial effect of nano based intracanal medications (Triple antibiotic-loaded chitosan nanoparticles and chlorohexidine loaded by silver nanoparticles) on Enterococcus faecalis count reduction in secondary endodontic infection cases.
Eligibility criteria
This trial accepts healthy volunteersQualifiers
Single rooted, single canal premolar teeth.
Teeth indicated for non-surgical root canal retreatment.
Teeth have signs and/or symptoms of post-treatment disease manifested by recurrent acute and/or chronic periapical abscess.
Teeth have clinical signs and symptoms of endodontic failure as sensitivity to percussion, pain, swelling or fistula.
Disqualifiers
Patients with any systemic diseases.
Pregnant or lactating patients.
Immunocompromised patients.
Non-restorable tooth.
Trial design
Design model
Parallel
Treatments tested in this trial
antimicrobial efficacy of nano based intracanal medications on Enterococcus faecalis count reduction in secondary endodontic infection cases.
Other interventionThe paper points of three samples (S1, S2, and S3) will be placed each in a sterile falcon tube containing 1ml of brain heart infusion and labeled according to the type of intracanal medication used. Samples will be homogenized by the vortex and and one micron of each sample will be cultured on Bile Esculin Agar for E. faecalis. The plate will be labeled as the tube of the same sample and incubated at 37οC for 24-48 h and then after incubation, the total numbers of E.faecalis will be counted and calculated to get the colony forming units (CFU/ml).
Treatment groups
30 Participants
are divided into 3 treatment groupsGroup A: Group 1: Chlorohexidine (CHX) gel.Active comparator 1 intervention
Group B: Group 2: Chlorohexidine loaded by silver nanoparticles (Ag-np) gel.Active comparator 1 intervention
Group C: Group 3: Triple antibiotic-loaded chitosan nanoparticles (tachnp) gel.Active comparator 1 intervention
Trial outcomes
Primary outcomes
1
the total numbers of Enterococcus Faecalis bacteria on bile esculin agar
colony forming units (CFU/ml).
Time frame
one weak after application of intracanal medicationSecondary outcomes
1
Post operative pain
pain on his own words
Time frame
one weak after application of intracanal medicationSponsors and contacts
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Source ClinicalTrials.gov